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Multilayer sensor used for detecting tea polyphenol, and production method and application of sensor

A technology of sensor and tea polyphenols, which is applied in the field of detection of tea polyphenols multilayer sensor and its preparation, can solve the problems of unguaranteed batch repeatability, uncontrollable infiltration amount, and increased operation complexity, etc., achieving low cost, The effect of high accuracy and low detection limit

Inactive Publication Date: 2016-11-09
CHONGQING UNIV
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Problems solved by technology

[0003] CN201510688617.1 discloses the "tea polyphenol content rapid detection test paper and its preparation method", which has the following problems: using ferrous iron as a color developing agent, the reagent itself is unstable and easily oxidized; the loading method of each layer of the test paper is direct infiltration , the amount of infiltration cannot be controlled, which is not only a waste of reagents, but also cannot guarantee the repeatability between batches; during the preparation process, the test paper needs to be freeze-dried, which increases the cumbersome operation; finally, only half of the polyphenols are obtained. Quantitative results

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  • Multilayer sensor used for detecting tea polyphenol, and production method and application of sensor
  • Multilayer sensor used for detecting tea polyphenol, and production method and application of sensor
  • Multilayer sensor used for detecting tea polyphenol, and production method and application of sensor

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Embodiment Construction

[0022] The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings.

[0023] 1. Multilayer sensor preparation and solution configuration

[0024] see figure 1 , the preparation process of the multi-layer sensor is as follows, first cut the qualitative filter paper used in the experiment into 2.0cm×2.0cm squares, soak in absolute ethanol for 12-15h, and dry at room temperature; then 10μL of tetrabutyl titanate solution Add dropwise to the center of the paper with a pipette, the solution spreads quickly on the paper, and dry at room temperature; then add 10 μL of dye solution, dry at room temperature, then add 10 μL of chitosan solution and 10 μL of sodium alginate solution; finally add dropwise 10 μL tetrabutyl titanate solution, so that one cycle of the multilayer sensor is completed. Repeat the above steps 5 times to build a multi-layer structure.

[0025] Wherein, the tetrabutyl titanate solution was p...

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Abstract

The invention provides a multilayer sensor used for detecting tea polyphenol, a production method of the multilayer sensor, and a method for detecting the tea polyphenol in tea leaves by using the sensor. The sensor used for rapidly detecting the tea polyphenol in the tea leaves has the advantages of low cost, realization of visual detection due to difference of the color change with different detected tea polyphenol concentrations, low detection limit, no need of a large device, no pretreatment of a sample, simplicity in operation, and good stability, and can be used a cheap, convenient, simple and effective electron tongue for real time discrimination of plant-derived beverages with different brands or qualities. The sensor allows the tea polyphenol to be directly rapidly detected without pre-treating the detected sample.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a multilayer sensor for detecting tea polyphenols, a preparation method thereof and an application of the sensor. Background technique [0002] Natural antioxidant polyphenols can participate in the metabolic reactions of the human body and prevent oxidative damage caused by free radicals. Through the accurate determination of antioxidant activity and polyphenol components, it has become relevant to achieve an objective evaluation of the physiological functions of various plant-derived foods and beverages. One of the hottest research hotspots in the research field. Among them, the Folin phenol colorimetric method is a classic method for detecting the content of total polyphenols. However, due to the interference of other reducing agents such as amino acids, ascorbic acid, and other sugars, the reproducibility is poor, which greatly hinders its wide application. From the ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/01
CPCG01N21/78G01N21/01
Inventor 侯长军付贝贝霍丹群杨眉
Owner CHONGQING UNIV
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