Eye mask and preparation method thereof
An eye mask and hydrogel technology, applied in the field of eye mask and its preparation, can solve the problems of insufficient extension, infection of animal epidemics, appearance of fine lines on the eyes, etc., to relieve eye fatigue, remove dark circles, and have high absorption efficiency. Effect
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[0047] The invention provides a method for preparing eye mask, comprising:
[0048] Mix chrysanthemum extract, wolfberry extract, menthol, stem cell growth factor powder and hydrogel to obtain eye mask solution;
[0049] The eye mask solution is prepared into an eye mask to obtain an eye mask.
[0050] The present invention preferably dissolves chrysanthemum extract and wolfberry extract in water to obtain a mixed solution; dissolves stem cell growth factor powder and menthol in the mixed solution to obtain a mixture; mixes the mixture with hydrogel to obtain an eye mask liquid. In the present invention, the mixture and the hydrogel are preferably mixed in an ice bath to obtain an eye mask solution. In the present invention, the mass concentration of the chrysanthemum extract in the mixed solution is preferably 2 mg / mL-10 mg / mL, more preferably 4 mg / mL-8 mg / mL, most preferably 5 mg / mL-6 mg / mL. In the present invention, the mass concentration of wolfberry extract in the mixe...
Embodiment 1
[0059] The serum-free medium containing mesenchymal stem cells was inoculated on a petri dish for cell culture at a seeding density of 1.0×10 5 cell / ml;
[0060] Replace the culture medium once every 3 days, subculture with more than 80% confluence, and collect the culture medium of the 3rd to 5th generation cells;
[0061]Filter the obtained cell culture solution through a 0.45 μm filter membrane to remove large particles, then filter the obtained filtrate through a 0.22 μm filter membrane to remove small particles below 3KDa, and use a Slice200 tangential flow device to filter the obtained filtrate for 3 hours Concentration to obtain a concentrated solution with a protein mass concentration of 1000mg / L;
[0062] The obtained concentrate is frozen on a lyophilizer to obtain stem cell growth factor powder.
Embodiment 2
[0064] Soak the polyvinyl alcohol in triple distilled water, reflux at a constant temperature of 95°C for 3 hours until the polyvinyl alcohol is completely dissolved to obtain a solution;
[0065] After the lysate obtained is cooled, it is stirred with water-soluble chitosan solution and glycerol mechanically to make it evenly mixed to obtain a mixed solution; the mass concentration of chitosan in the mixed solution is 2%, and the mass concentration of glycerin is 1%. The mass concentration of polyvinyl alcohol is 7%;
[0066] The obtained mixed solution was defoamed by ultrasonic cleaning for 15 minutes, and the defoamed mixed solution was introduced into an irradiation tube, and irradiated with light with an intensity of 8KGry for 4.5 hours under a nitrogen atmosphere to obtain a hydrogel.
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