Scar-removing composition and dressing
A composition and scar removal technology, applied in the field of biomedicine, can solve problems such as skin damage
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[0063] Example 1
[0064] 1. Collection of stem cell conditioned medium
[0065] Take the P3 generation of umbilical cord mesenchymal stem cells and culture them with DMEMF12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.15 mL / cm 2 After culturing the phenol red 1640 statically for 72 hours, the conditioned medium is collected, and the collected conditioned medium is centrifuged at 300g for 5 minutes, the supernatant is taken, and the obtained is the stem cell conditioned medium for use.
[0066] 2.3D modeling
[0067] Use the built-in laser of the 3D printer to scan the surface of the patient’s scar to obtain the size, shape, thickness and thickness of the wound and the thickness of the epidermis of the patient’s normal skin, and then use the PlyEdit software to process the scanned image to eliminate digital noise and other processing. Edit to produce a continuous surface imag...
Example Embodiment
[0092] Example 2
[0093] 1. Collection of stem cell conditioned medium
[0094] Take the P3 generation of umbilical cord mesenchymal stem cells and culture them with DMEMF12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 90%, remove the culture supernatant, wash twice with PBS, and add 0.1 mL / cm 2 After culturing the phenol red 1640 statically for 72 hours, the conditioned medium is collected, and the collected conditioned medium is centrifuged at 200g for 5 minutes, the supernatant is taken, and the obtained is the stem cell conditioned medium for use.
[0095] 2.3D modeling
[0096] Use the built-in laser of the 3D printer to scan the surface of the patient’s scar to obtain the size, shape, thickness and thickness of the wound and the thickness of the epidermis of the patient’s normal skin, and then use the PlyEdit software to process the scanned image to eliminate digital noise and other processing. Edit to produce a continuous surface image...
Example Embodiment
[0104] Example 3
[0105] 1. Collection of stem cell conditioned medium
[0106] Take the P3 generation of umbilical cord mesenchymal stem cells and culture them with DMEMF12+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.2mL / cm 2 After culturing the phenol red 1640 statically for 72 hours, the conditioned medium is collected, and the collected conditioned medium is centrifuged at 400g for 5 minutes, the supernatant is taken, and the obtained is the stem cell conditioned medium for use.
[0107] 2.3D modeling
[0108] Use the built-in laser of the 3D printer to scan the surface of the patient’s scar to obtain the size, shape, thickness and thickness of the wound and the thickness of the epidermis of the patient’s normal skin, and then use the PlyEdit software to process the scanned image to eliminate digital noise and other processing. Edit to produce a continuous surface image;...
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