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Method for extracting tabersonine from Voacanga Africana seeds

A technology of Falcone seeds and its boning, applied in the field of boning extraction, can solve the problems of large amount of waste water, high cost, long time consumption, etc., and achieve the effects of high purity, short time consumption and simple operation.

Inactive Publication Date: 2016-12-21
成都合盛生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a method for extracting Tabonin from Fukang tree seeds, which can effectively solve the problems of using toxic solvents, high cost, and waste water produced in the existing extraction process. Large amount, low yield, long time-consuming and other problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of method extracting it Boning from vulcan tree seed, comprises the following steps:

[0025] (1) Pulverization: crush 1 kg of dried Fukang tree seeds, pass through a 20-mesh sieve to obtain powder, and quickly grind the powder with liquid nitrogen into a fine, smooth and non-rough ultrafine powder;

[0026] (2) Enzymolysis: quickly add the ultrafine powder obtained in step (1) into the cellulose enzyme solution with an enzyme concentration of 0.5g / L, the enzymolysis temperature is 40°C, and the enzymolysis time is 2 hours to obtain an enzymolysis solution;

[0027] (3) Supercritical extraction: filter the enzymolysis solution obtained in step (2), remove the filtrate, dry the obtained solid at 40°C, then put the dried material into an extraction kettle, and extract with supercritical carbon dioxide at a temperature of 40°C , the extraction pressure is 28MPa, the carbon dioxide flow rate is 25L / h, flow extraction after 30 minutes of equilibrium, the extraction ti...

Embodiment 2

[0033] A kind of method extracting it Boning from vulcan tree seed, comprises the following steps:

[0034] (1) Pulverization: Take dried Fukang tree seeds and pulverize 1kg, pass through a 40-mesh sieve to obtain a powder, and quickly grind the powder with liquid nitrogen into a fine, smooth and non-rough ultra-fine powder;

[0035] (2) Enzymolysis: quickly add the ultra-fine powder obtained in step (1) into the cellulose enzyme solution with an enzyme concentration of 0.7g / L, the enzymolysis temperature is 45°C, and the enzymolysis time is 3 hours to obtain an enzymolysis solution;

[0036] (3) Supercritical extraction: filter the enzymolysis solution obtained in step (2), remove the filtrate, dry the obtained solid at 45°C, then put the dried material into an extraction kettle, and extract with supercritical carbon dioxide at a temperature of 50°C , the extraction pressure is 30MPa, the carbon dioxide flow rate is 30L / h, flow extraction after 20 minutes of equilibrium, the ...

Embodiment 3

[0042] A kind of method extracting it Boning from vulcan tree seed, comprises the following steps:

[0043] (1) Pulverization: Take dried Fukang tree seeds and pulverize 1kg, pass through a 40-mesh sieve to obtain a powder, and quickly grind the powder with liquid nitrogen into a fine, smooth and non-rough ultra-fine powder;

[0044] (2) Enzymolysis: quickly add the ultrafine powder obtained in step (1) into the cellulose enzyme solution with an enzyme concentration of 0.6g / L, the enzymolysis temperature is 40°C, and the enzymolysis time is 2.5h to obtain an enzymolysis solution;

[0045](3) Supercritical extraction: filter the enzymolysis solution obtained in step (2), remove the filtrate, dry the obtained solid at 45°C, then put the dried material into an extraction kettle, and extract with supercritical carbon dioxide at a temperature of 40°C , the extraction pressure is 28MPa, the carbon dioxide flow rate is 28L / h, flow extraction after 30 minutes of equilibrium, the extra...

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Abstract

The invention discloses a method for extracting tabersonine from Voacanga Africana seeds. The method comprises the following steps: (1) crushing: crushing the dried Voacanga Africana seeds, screening the crushed seed by a 20-40 mesh screen to obtain powder, grinding the powder by liquid nitrogen to obtain superfine powder; (2) enzymolysis: adding the superfine powder obtained in the step (1) into a cellulase liquid with the enzyme concentration of 0.5-0.7 g / L, allowing the mixture to perform enzymolysis reaction for 2-3 hours at 40-45 DEG C, so as to obtain enzymatic hydrolysate; (3) carrying out supercritical extraction; (4) carrying out alcohol extraction; (5) carrying out separating and purifying. According to the extraction method, toxic solvents such as benzene and chloroform are not used, and no large amount of waste water is generated, and the environment is not polluted, meanwhile, the extraction method is simple to operate, short in time consumption, high in yield and purity, the yield is up to 3.9%, and the purity is 99.6%.

Description

technical field [0001] The invention belongs to the technical field of extracting tabonin, and in particular relates to a method for extracting tabonin from the seeds of Fukang tree. Background technique [0002] Voacango Africana Stapf, also known as African potato fruit, is the seed of Voacango Africana Stapf, a plant of the family Apocynaceae from Africa, which contains a variety of alkaloids. [0003] Tabersonine, also known as Willow Leaf Glycyrrhizin, CAS NO.4429-63-4, molecular formula C 21 h 24 N 2 o 2 , with a molecular weight of 336.42, is the most important alkaloid component in the seeds of Fukang tree. It has antihypertensive, antitumor, hypoglycemic, diuretic and other effects, and can be used to treat stroke sequelae, ischemic hypertensive encephalopathy, depression, anxiety, emotional instability and other diseases caused by cerebrovascular disease, and is suitable for premature aging. Symptoms of dementia are eliminated. It is now mainly used as a semi...

Claims

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Application Information

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IPC IPC(8): C07D471/20
CPCC07D471/20Y02P20/54
Inventor 刘海峰姚晨张彩玲
Owner 成都合盛生物技术有限公司
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