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Preparation of placenta sub-totipotent stem cell culture medium for skin care

A sub-totipotent stem cell and stem cell culture technology, which is applied in the field of preparation of placental sub-totipotent stem cell culture medium, can solve the problems of low biological activity of functional ingredients, many residual impurities, skin damage, etc., to improve skin brightness, enhance absorption effect, and guarantee safety effect

Inactive Publication Date: 2017-01-04
BEIJING HEALTH & BIOTECH (H&B) CO LTD
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AI Technical Summary

Problems solved by technology

In view of the broad application prospects of placental sub-totipotent stem cells, most of the skin care products currently on the market are fine chemical products, and there are many impurities in the initial raw materials. The biological activity of the functional ingredients themselves is low and difficult to maintain, and the effect of improving skin physiological state is not good. placental subtotipotent stem cells can be used in skin care products

Method used

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specific Embodiment

[0036] A preparation of placental subtotipotent stem cell culture fluid for skin care and maintenance, specifically comprising the steps of:

[0037] (1) The collected human placenta tissue was cut and broken into 3 Small pieces of placental tissue;

[0038] (2) Digest and degrade small pieces of placental tissue with 5 mg / mL trypsin at 37°C for 30 minutes, and shake gently 5 times during the degradation period. After the degradation is completed, the cell suspension is collected by centrifugation through a cell sieve.

[0039] (3) The cell suspension was separated by magnetic cell sorting, first using CD184 + Magnetic beads for negative selection to obtain CD184 - cells, followed by CD151 - Magnetic bead separation to obtain CD151 + CD184 - cells, finally with Oct4 - Magnetic beads isolate the target cells, namely placental subtotipotent stem cells;

[0040] (4) Configuration of stem cell culture medium: mix 7L of serum-free phenol red-free DMEM / F12 culture medium with ...

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Abstract

The invention discloses a preparation method of a placenta sub-totipotent stem cell culture medium for skin care. Human placenta tissue is cut, smashed, degraded, screened and separated to obtain placenta sub-totipotent stem cells; then the placenta sub-totipotent stem cells are placed in a stem cell culture medium containing amino acid, non-essential amino acid and cell growth factors to be cultured; finally, collected supernate is filtered, small-molecular collagen peptide is added, and the placenta sub-totipotent stem cell culture medium can be obtained. The placenta sub-totipotent stem cell culture medium prepared through the method is high in active substance content and high in bioactivity, can care skin, can promote skin regeneration, and enhances metabolism of skin.

Description

technical field [0001] The invention relates to the technical field of biological cosmetics, in particular to the preparation of a placental subtotipotent stem cell culture solution for skin care and maintenance. Background technique [0002] Stem cells are a kind of progenitor cells with the characteristics of self-replication and multilineage differentiation. Stem cells are the basic structural and functional units of the human body, so it can be seen that stem cells are the origin cells of various tissues and organs of the human body. During the whole process of the human body from fertilized egg to fetus, and from fetus to adulthood, stem cells gradually lose their totipotency, and form sub-totipotent stem cells, pluripotent stem cells and multipotent stem cells from totipotent embryonic stem cells; these are at different developmental stages Stem cells play a decisive role in the development, growth, damage, and repair of human tissues and organs. Therefore, it can be ...

Claims

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Application Information

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IPC IPC(8): C12N5/0735A61K8/98A61Q19/00A61Q19/02
Inventor 杨振亚韩之波
Owner BEIJING HEALTH & BIOTECH (H&B) CO LTD
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