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Reagents, detection methods and applications for h5n6 avian influenza virus detection

An avian influenza virus and reagent technology, which is applied in the field of H5N6 avian influenza virus detection to avoid the spread of the epidemic and ensure social security.

Active Publication Date: 2020-01-07
SHENZHEN ACAD OF INSPECTION & QUARANTINE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently there is no effective detection reagent and method for H5N6 avian influenza virus

Method used

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  • Reagents, detection methods and applications for h5n6 avian influenza virus detection
  • Reagents, detection methods and applications for h5n6 avian influenza virus detection
  • Reagents, detection methods and applications for h5n6 avian influenza virus detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] 1. Establishment and optimization of the reaction system

[0029] Use the artificially synthesized viral plasmid of H5N6 avian influenza virus as the positive standard, and use commercial RNA extraction kits or Trizol nucleic acid extraction reagents to extract viral genome RNA for other samples, and store them at -20°C after aliquoting spare. Other samples for testing include: H5N1 inactivated strains, swine influenza classic H1N1 strains, swine influenza classic H3N2 strains and avian influenza H7, H9 standard detection antigens. Preserved by Plant Inspection and Quarantine Technical Center. In this example, specific detection primers and probes were designed for the genomic RNA of H5N6 avian influenza virus. The specific sequences of the primers and probes are shown in Table 1.

[0030] Table 1 Primer and Probe Sequences

[0031]

[0032]

[0033] In this example, the primer concentration and probe concentration were optimized, as follows:

[0034] (1) Opti...

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PUM

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Abstract

The invention discloses a reagent used for H5N6 avian influenza virus detection, a detection method and application. The reagent used for H5N6 avian influenza virus detection comprises a primer pair and a probe, wherein the probe is in the amplification target region of the primer pair; a forward primer of the primer pair is a sequence shown in Seq ID No.1; a reverse primer of the primer pair is a sequence shown in Seq ID No.2; the probe is a sequence shown in Seq ID No.3 or a reverse complementary sequence of the sequence shown in Seq ID No.3. The reagent used for H5N6 avian influenza virus detection has strong specificity toward the avian influenza subspecies H5N6, can specifically detect H5N6 from numerous avian influenza viruses accurately and sensitively, lays the foundation for rapid diagnosis of emergency epidemics and rapid detection of large-scale samples, is especially suitable for inspection and quarantine departments and other departments to use and provides a powerful science tool for guaranteeing social security and avoiding epidemic spreading and diffusion.

Description

technical field [0001] The present application relates to the field of detection of avian influenza virus, in particular to a reagent, detection method and application for detection of H5N6 avian influenza virus. Background technique [0002] Avian influenza is a zoonotic disease caused by a virus that usually only infects birds. The symptoms after human infection are mainly high fever, cough, runny nose, myalgia, etc. Most of them are accompanied by severe pneumonia. In severe cases, heart, kidney and other organ failures lead to death. It can enter the human body and infect humans through the digestive tract and respiratory tract. Humans can also be infected by direct contact with poultry infected by the avian influenza virus and its feces or direct contact with the avian influenza virus. It is also the way of transmission through droplets and contact with respiratory secretions. If you directly contact items with a considerable amount of viruses, such as poultry feces, f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/701C12Q2563/107C12Q2561/113
Inventor 孙洁廖立珊陶虹陈兵秦智锋卢奕良曾少灵马岚卢体康刘建利郑晓聪唐金明林庆燕陈书琨詹爱军兰文升曹琛福王津津
Owner SHENZHEN ACAD OF INSPECTION & QUARANTINE
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