Immunochromatography test paper for quickly detecting fenitrothion based on upconversion fluorescent nanoparticles and preparation method thereof

A nanoparticle and test paper technology, applied in the field of chemical identification, achieves the effects of low toxicity, improved signal-to-noise ratio and high luminous intensity

Inactive Publication Date: 2017-01-04
DALIAN NATIONALITIES UNIVERSITY
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Problems solved by technology

However, the use of up-conversion nano-fluorescent materials as markers, combined with immu

Method used

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  • Immunochromatography test paper for quickly detecting fenitrothion based on upconversion fluorescent nanoparticles and preparation method thereof
  • Immunochromatography test paper for quickly detecting fenitrothion based on upconversion fluorescent nanoparticles and preparation method thereof
  • Immunochromatography test paper for quickly detecting fenitrothion based on upconversion fluorescent nanoparticles and preparation method thereof

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Embodiment Construction

[0037] In recent years, due to the optical properties of rare earth up-conversion nanomaterials, their applications in the biological field have a lot of untapped potential. β-NaYF 4 :Yb 3+ ,Er 3+ (Tm 3+ ) is the material with the highest up-conversion efficiency found so far, so it has attracted extensive attention in biological detection and labeling. However, since the prepared rare earth up-conversion nanomaterials are oil-soluble materials, it is necessary to use KMnO 4 / NaIO 4 Convert oil-soluble nanoparticles to water-soluble nanoparticles.

[0038] The present invention utilizes high-temperature pyrolysis method to prepare β-NaYF coated with oleic acid 4 :Yb 3+ ,Er 3+ Nanoparticles.

[0039] 1. Preparation of green rare earth up-conversion nanomaterials

[0040] (1) Experimental instruments and experimental reagents

[0041] Table 1 List of experimental instruments

[0042]

[0043] Table 2 List of chemical reagents

[0044]

[0045] (2) Preparation ...

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Abstract

The invention relates to immunochromatography test paper for quickly detecting fenitrothion based on upconversion fluorescent nanoparticles and a preparation method thereof and belongs to the technical field of chemical identification. The pesticide test paper comprises a test paper plate, a sample dropwise-adding unit, a detection unit and a sample recycling unit. The sample dropwise-adding unit, the detection unit and the sample recycling unit are connected to the test paper plate. The detection unit is located in the middle of the test paper plate. The detection unit comprises antigen coating buffer and confining liquid. The immunochromatography test paper has the advantages that the test paper has the advantages of quickness, simplicity, convenience, specificity, sensitivity and low cost; and particularly the test paper has the characteristics of quickness, simplicity and convenience, is particularly suitable for use by vast grassroots units and field operation workers and massive tightly-scheduled detection and large-area census and the like, has huge development potential and application prospect, is taken as one of most promising novel technologies in food safety detection and has become the main development trend in food safety detection.

Description

technical field [0001] The invention relates to the technical field of chemical identification, in particular to an immunochromatographic test paper for rapidly detecting fenitrothion based on up-conversion fluorescent nanoparticles and a preparation method thereof. Background technique [0002] In the early 1980s, a rapid detection technology emerged in western developed countries. The material corresponding to the binding pad is labeled, and after the action of the nitrocellulose membrane, the antibody and the antigen are specifically combined here, and this technology is called the immunochromatography test paper detection technology. These substances used for labeling all have the color or fluorescence that can be seen by the naked eye or under the excitation of ultraviolet light to see the specific binding of the antibody to the antigen. Immunochromatographic test strips are used in more and more fields because of their fastness, convenience, and simple operation, and ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531G01N33/532G01N21/64
CPCG01N21/6428G01N33/531G01N33/532G01N33/558G01N2021/6439
Inventor 华瑞年国婷婷张伟于基成那立艳
Owner DALIAN NATIONALITIES UNIVERSITY
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