A drug for mirror image pain caused by cancer invasion
A drug and mirror image technology, applied in the direction of drug combinations, antipyretics, pharmaceutical formulas, etc., can solve the problems of unclear MIP, unsatisfactory treatment effect, uncertain MIP treatment effect, etc., and achieve the effect of great application value
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Embodiment 1
[0025] Continuous operation is involved in embodiment 1 and embodiment 2, so the number of test days is counted continuously.
[0026] The structural formula of ligustrazine hydrochloride is as follows:
[0027]
[0028] The structural formula of sinomenine hydrochloride is as follows:
[0029]
[0030] Ligustrazine can be extracted from Ligusticum chuanxiong or obtained by chemical synthesis. Common extraction methods are as follows: Rhizoma Chuanxiong is extracted with ethanol by ultrasound, and the extract is concentrated and dried. Ligustrazine can also be replaced by a salt of Ligustrazine, such as Ligustrazine hydrochloride, Ligustrazine phosphate, etc.
[0031] Sinomenine can be extracted from Qingfengteng. The common extraction methods are as follows: 1. Alcohol extraction of sinomenes, acid soluble, extraction, and water recrystallization to obtain the product; 2. Sinomenization, benzene extraction, crystallization, decolorization, and water recrystallization...
Embodiment 2
[0038] Example 2, the effect of CQ compound on cancer invasion mirror pain
[0039] 1. Preparation of CQ compound
[0040] The CQ compound consists of ligustrazine hydrochloride and sinomenine hydrochloride, and the mass ratio of ligustrazine hydrochloride to sinomenine hydrochloride is 0.8:1. Take the CQ compound and dissolve it in ultrapure water to obtain a 40mg / mL stock solution, then dilute it with normal saline to obtain the CQ compound solution.
[0041] 2. Drug administration in groups
[0042] Model group (Model, n=12): the subjects were the model mice obtained in Example 1, and each of the 8th day, the 9th day and the 10th day of the test was intraperitoneally injected once with normal saline, and the single injection dose was 0.4mL normal saline / 20g mouse;
[0043] CQ compound high-dose group (H-CQ, 200mg / kg, n=12): the object is the model mouse that embodiment 1 obtains, test the 8th day, the 9th day and the 10th day each intraperitoneal injection of CQ compoun...
Embodiment 3
[0070] Example 3. Amantadine hydrochloride enhances the analgesic effect of CQ compound on MIP
[0071] 1. Preparation of model mice
[0072] SPF grade BALB / c male mice were pre-stimulated for 3 consecutive days before the test (once a day, the hind feet of both sides of the mice were stimulated with quantitative VonFrey filaments each time). On the first day of the experiment, mice whose bilateral hindfoot mechanical pain thresholds were ≥0.4 g were taken, and each mouse was injected with 0.2 mL of S180 tumor strain cell solution (viable cell concentration 1×10 7 pieces / ml). On the 5th to 7th day of the test, the mice in the Surgery group whose mechanical pain thresholds on both the cancer invasion side and the mirror image side were ≤0.07 g were regarded as model mice.
[0073] Two, preparation of CQ compound
[0074] The CQ compound consists of ligustrazine hydrochloride and sinomenine hydrochloride, and the mass ratio of ligustrazine hydrochloride to sinomenine hydroch...
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