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A method for biodegrading lycopene to generate 6-methyl-5-hepten-2-one and isooctadienone

A technology for isooctadienone and lycopene, which is applied in the field of biodegradation of lycopene and achieves the effects of good social and economic benefits, mild reaction conditions and improved degradation rate

Active Publication Date: 2017-12-05
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report that it can degrade lycopene to produce 6-methyl-5-hepten-2-one and isooctadienone

Method used

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  • A method for biodegrading lycopene to generate 6-methyl-5-hepten-2-one and isooctadienone
  • A method for biodegrading lycopene to generate 6-methyl-5-hepten-2-one and isooctadienone
  • A method for biodegrading lycopene to generate 6-methyl-5-hepten-2-one and isooctadienone

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] First configure the fermentation medium for lycopene degradation: yeast powder 3g, peptone 10g, NaCl 10g, glucose 1g, distilled water 1L, adjust the pH to 7.0, put each 100mL fermentation medium in a 250mL Erlenmeyer flask, seal Afterwards, sterilize at a high temperature of 121°C for 20 minutes, and cool to room temperature for use.

[0035] Preparation of lycopene solution: Disperse 200 mg of lycopene in 2 g of Tween 80 to obtain a lycopene dispersion, then dissolve the lycopene dispersion in 400 mL of dichloromethane, and dissolve the dichloromethane Evaporate under reduced pressure, and dissolve the residue after decompression evaporation in 10 mL of ethanol; pass the lycopene solution through a 0.45 μm organic filter membrane to remove internal bacteria, and obtain the product.

[0036] Biodegradation process: Add the lycopene solution to multiple fermentation media respectively, until the final concentration of lycopene is 100, 200, 300, 400, 500, 600 mg / L respect...

Embodiment 2

[0051] The degradation products of lycopene fermented and degraded by Bacillus cereus of the present invention are measured, the specific method is:

[0052] The fermentation broth was centrifuged at high speed (4°C, 6500r / min, 30min) under dark conditions, and the separated supernatant was taken out, and an equal volume of dichloromethane was added to extract repeatedly 3 times to obtain the lycopene fermentation extract. Add anhydrous Na 2 SO 4 Dry overnight, concentrate in vacuo to 1 mL, and perform GC-MS qualitative analysis.

[0053] Gas phase conditions: Chromatographic column: HP-5 (30m×0.25mm; 0.25μm); heating program: initial temperature of 45°C, keep for 5min, rise to 110°C at 4°C / min, hold for 5min, rise to 1°C / min 150°C, hold for 5min, rise to 200°C at 3°C / min, rise to 280°C at 5°C / min, hold for 5min; inlet temperature: 270°C; transfer line temperature: 270°C; split ratio: 1:1; Carrier gas: He gas; flow rate: 1mL / min.

[0054] Mass spectrometry conditions: ioni...

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Abstract

The invention discloses a method for generating 6-methyl-5-hepten-2-one and isooctyl dienone through biodegradation of lycopene. According to the method, the 6-methyl-5-hepten-2-one and isooctyl dienone is generated through the degradation of lycopene by virtue of bacillus cereus. In the method provided by the invention, the lycopene is degraded by virtue of the bacillus cereus for the first time so as to generate flavoring substances, namely the 6-methyl-5-hepten-2-one and the isooctyl dienone, so that a new way for generating the flavoring substances through the microbial degradation of the lycopene is created, and the method has the characteristics of being mild in reaction conditions, easy for separation, green and environment-friendly and the like. By comparing degrading effects of different substrate concentrations, it discovers that an optimum lycopene degrading effect is achieved when the concentration of the lycopene in a fermentation medium is 300-500mg / L.

Description

technical field [0001] The invention relates to a method for biodegrading lycopene, in particular to a method for using Bacillus cereus to degrade lycopene to generate 6-methyl-5-hepten-2-one and isooctadienone, belonging to Microbial fermentation technology field. Background technique [0002] Lycopene is an oxygen-free carotenoid, also known as ψ-carotene, which is called lycopene because it was first isolated from tomato. Lycopene mainly exists in the ripe fruit of tomato, a plant of the family Solanaceae, and is the precursor of many aroma substances. For example, phytoene can be degraded into aromatic substances such as solanone, methyl octenone and its derivatives, geranyl acetone or farnesyl acetone, which are the source of plant floral and fruity aromas, And the aroma threshold is very low, which has important value in the preparation of flavors and fragrances. [0003] There are three ways to degrade lycopene, namely physical degradation, thermal cracking, chemic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/26C12R1/085
CPCC12P7/26
Inventor 杨雪鹏孙建宏叶建斌汪芳芳马科毛多斌
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY