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Determination method of biodegradable humic acid in leachate

A determination method and leachate technology, applied in the field of environmental science, can solve the problems of secondary environmental pollution, a large number of chemical reagents, and long processing time, and achieve the effects of no environmental pollution, simple pretreatment, and fast detection speed.

Active Publication Date: 2019-05-31
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, the method for determining the content and composition of biodegradable humic acid in leachate is mainly realized through the extraction and composition analysis of humic acid in leachate. Reagents, causing secondary environmental pollution

Method used

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  • Determination method of biodegradable humic acid in leachate
  • Determination method of biodegradable humic acid in leachate
  • Determination method of biodegradable humic acid in leachate

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Embodiment 1

[0039] In the landfill leachate treatment workshop, 20 leachate samples were collected from the adjustment tank, after anaerobic treatment, after aerobic treatment, and during different periods of the treatment process. After centrifugation, the supernatant was collected, and the supernatant was centrifuged and passed through a 0.45 μm pore size filter. Filter the membrane, collect the filtrate, divide each of the above filtrate samples into two, one part is freeze-dried into powder, and the other part is stored at 4°C, and the infrared spectrum of the leachate organic solid powder is measured with an infrared spectrometer, and then its data is converted into Absorption spectrum patterns, saved sequentially in a file in csv data format. In addition, dilute the leachate sample stored at 4°C with ultrapure water so that it does not exceed two-thirds of the range of the fluorescence spectrometer, and scan the synchronous fluorescence spectrum with a wavelength difference of 30nm a...

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Abstract

A measurement method of biodegradable humic acid in leachate includes the steps of: collecting leachate samples in different bio-treatment steps; extracting and purifying leachate organic substances, and scanning the leachate organic substances to obtain infrared spectrum and fluorescent spectrum thereof; determining the number of benzene rings in and the content of the biodegradable humic acid according to parallel factor analysis of three-dimensional fluorescence spectrum of the leachate organic substances; and determining functional group composition characters of the biodegradable humic acid in the leachate through correlation analysis on synchronous fluorescent spectrum and infrared spectrum of the leachate organic substances. The method can be used for determining the composition and relative content of the biodegradable humic acid in the leachate, is simple in sample pre-treatment and has high analysis speed, and can provide the basis for technical optimization and scheme selection of treatment technology of the leachate.

Description

technical field [0001] The invention belongs to the field of environmental science and technology, and in particular relates to a method for determining biodegradable humic acid in leachate. Background technique [0002] Landfill leachate comes from the process of stacking, composting and landfilling of garbage. It contains small molecular organic acids, humic acids, polysaccharides and other substances. It is a very difficult organic wastewater to treat. The most important substance affecting the biodegradability of landfill leachate is humic acid, which is a complex molecule synthesized from lignin degradation intermediates and amino acids. The most basic structural unit is a benzene ring, which has various a substituent. Humic acid in leachate is a mixture of a large class of organic molecules, and the composition and biodegradability of different humic acids are quite different: some humic acids have low humification degree and unstable structure, and can be degraded by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/3577
CPCG01N21/3577G01N21/64
Inventor 何小松席北斗张慧黄彩红高如泰檀文炳祖国峰
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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