Biological fermented feed and preparation method thereof

A bio-fermented feed, solid fermentation technology, applied in the field of bio-fermented feed and its preparation

Active Publication Date: 2017-02-08
北京康缘益生生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Cassava residue is rich in carbohydrates, of which the nitrogen-free extract reaches about 70%. Although it contains a large amount of crude fiber, the crude fiber is mainly composed of cellulose with relatively hig

Method used

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  • Biological fermented feed and preparation method thereof
  • Biological fermented feed and preparation method thereof
  • Biological fermented feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0196] 1. Activated bacteria

[0197] 1. Strains (purchased from the National Culture Collection Center): Aspergillus niger (ACCC32589), Bacillus licheniformis (ACCC19747), Candida utilis (ACCC20060), Bacillus subtilis (CGMCC 1.934), Bacillus coagulans (ACCC00399 ), Lactobacillus plantarum (ACCC03954)

[0198] 2. Preparation of solid medium slant for bacterial strain activation (weight ratio)

[0199] Under aseptic conditions, when the temperature of the culture medium has dropped to about 50°C, divide it into sterile empty test tubes, 25ml each, place at an inclination of 30°, and solidify. Blank culture at 37 ℃ for 24 hours, no bacteria growth, can be used. The slant medium formula of each strain is as follows:

[0200] (1) Fungus (Aspergillus niger): 30% potato, 20% glucose, 1.5% agar powder, the balance is pure water, pH=6.0. Sterilize at 120°C for 15 minutes.

[0201] (2) Yeast (Candida utilis): Wort (Baume degree=12), agar powder 1.5%. The balance is purified wate...

Embodiment 2

[0338] 1. Activated bacteria

[0339] 1. Strains (purchased from the National Culture Collection Center): Aspergillus niger (ACCC32589), Bacillus licheniformis (ACCC19747), Candida utilis (ACCC20060), Bacillus subtilis (CGMCC 1.934), Bacillus coagulans (ACCC00399 ), Lactobacillus plantarum (ACCC03954)

[0340] 2. Preparation of solid medium slant for bacterial strain activation (weight ratio)

[0341] Under aseptic conditions, when the temperature of the culture medium has dropped to about 50°C, divide it into sterile empty test tubes, 25ml each, place at an inclination of 30°, and solidify. Blank culture at 37 ℃ for 24 hours, no bacteria growth, can be used. The slant medium formula of each strain is as follows:

[0342] (1) Fungus (Aspergillus niger): 20% potato, 10% glucose, 1.5% agar powder, the balance is pure water, pH=6.0; sterilize at 120°C for 15 minutes.

[0343] (2) Yeast (Candida utilis): Wort (Baume degree=10), agar powder 1.5%, surplus are pure water, pH ...

Embodiment 4

[0465] Embodiment 4 fattening pig feeding effect test

[0466] 1. Experimental design: Single factor design is adopted. There were 4 groups (control group, treatment group 1, treatment group 2 and treatment group 3) in the test. The control group, treatment group 1, 2 and 3 added 0%, 20%, and 35% of the basal diet weight on the base material respectively. , 40% of the above-mentioned fermented feed of the present invention. Based on the air-dried matter, the dietary indicators of each group were basically the same except for the crude fiber level, digestible energy, protein, calcium, phosphorus and other indicators.

[0467] 2. Select 60 healthy and disease-free long-fatten pigs of the same breed, about 83.0kg, as experimental animals and diets, and randomly divide them into 4 groups according to the principle of similar body weight. Each group has 5 repetitions, and each repetition 3 pigs. The control group was fed with corn-wheat bran-soybean meal type conventional ration...

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Abstract

The invention relates to biological feed, in particular to a biological fermented feed and a preparation method thereof. The method consists of: subjecting Aspergillus niger, Bacillus licheniformis, Bacillus coagulans, Bacillus subtilis, Lactobacillus plantarum, and Candida utilis respectively to two-stage liquid fermentation culture, and then conducting three-stage solid fermentation culture to obtain fermented culture, i.e. biological feed. The fermented culture provided by the invention utilizes neural network, pattern recognition, genetic algorithm and other optimization methods to optimize the fermentation conditions, a biosynthesis network is constructed according to the principle of metabolic engineering, and pathway analysis and metabolic flux control are carried out on the synthetic network on the basis, and enzymolysis, anaerobism, aerobiosis, bacterium cultivation and other processes are employed, thus increasing the protein content, reducing the content of crude fiber, improving the palatability and fragrance, increasing probiotics, and removing toxins from feed raw materials.

Description

technical field [0001] The invention relates to a biological feed, in particular to a biological fermented feed and a preparation method thereof. Background technique [0002] Feed and grain production has always been a weak link in my country's national economy. Due to the influence of population growth, decrease of arable land and increase of meat consumption, the balance of grain supply and demand in my country is very fragile. my country's per capita grain has been below 400kg, of which about 40% of the total grain output is used for feed production. Under the long-term shortage of arable land and water resources, it is difficult to increase my country's grain output. Therefore, the shortage of feed resources that has long restricted the development of my country's agriculture and animal husbandry, especially the serious shortage of protein feed, cannot be effectively resolved in the short term. . [0003] The best way to meet people's growing demand for meat, poultry,...

Claims

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Application Information

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IPC IPC(8): A23K10/18C12N1/20C12N1/14C12N1/16C12R1/685C12R1/10C12R1/07C12R1/125C12R1/25C12R1/72
CPCC12N1/14C12N1/16C12N1/20
Inventor 张传军张晓红张中亲任存邦张宪德张亚洁
Owner 北京康缘益生生物科技有限公司
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