A strain of Serratia gorsii and its use

A technology for Serratia gasseri and inoculum, which is applied in the field of microorganisms and can solve the problems of pathogenic bacteria easily producing drug resistance, endangering the health of humans and animals, and decreasing control effect.

Active Publication Date: 2019-12-10
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of chemical agents, on the one hand, pathogenic bacteria are likely to develop drug resistance, thereby greatly reducing the control effect; on the other hand, it is easy to cause serious environmental pollution, endangering the health of humans and animals

Method used

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  • A strain of Serratia gorsii and its use
  • A strain of Serratia gorsii and its use
  • A strain of Serratia gorsii and its use

Examples

Experimental program
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Effect test

preparation example Construction

[0066] The preparation method of potato liquid medium is as follows: take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of distilled water, and boil for 30 minutes; collect the filtrate after gauze filtration, add 20.0 g of glucose to the filtrate, and distill the volume to 1.0 L, and then sterilized at 115°C for 15 minutes. There is no need to adjust the pH value of the potato liquid medium, that is, the pH value is natural.

[0067] The preparation method of potato liquid medium 1 is as follows: take 200 g of peeled potatoes, cut into small pieces, add 1.0 L of distilled water, and boil for 30 minutes; collect the filtrate after gauze filtration, add 20.0 g of glucose in the filtrate, and distill to volume with distilled water to 1.0L, adjust the pH to 4.0; then sterilize at 115°C for 15 minutes.

[0068] Potato liquid medium 2: In the preparation method of potato liquid medium 1, "adjust the pH value to 4.0" was replaced with "adjust the pH value to 5.0",...

Embodiment 1

[0074] Example 1. Isolation, identification and preservation of Serratia grimesii (Serratia grimesii) S-2CGMCC No.13034

[0075] 1. Separation

[0076] 1. Processing of soil samples

[0077] (1) Add 10 g of soil sample (collected from the potato soil in Guyuan County, Ningxia Hui Autonomous Region) and an appropriate amount of glass beads to 90 mL of sterile water, vibrate for 10 min, and let stand for 30 s to make a soil stock solution (at this time, the dilution is recorded as 10 -1 ). Take 1 mL of the supernatant of the soil stock solution and add it to a test tube filled with 9 mL of sterile water and mix well (the dilution is 10 at this time). -2 ), then draw 1mL from this test tube and add it to another test tube filled with 9mL sterile water and mix well, and so on to make dilution A (dilution is 10 -3 ) and diluent B (dilution of 10 -4 ).

[0078] 2. Take Streptomyces scab 4.76, inoculate it on an oat solid plate, and culture it statically at 28° C. for 10 days; t...

Embodiment 2

[0092] The propagation of embodiment 2, Serratia grisneri S-2

[0093] The specific steps for the propagation of Serratia gaseri S-2 are as follows:

[0094] 1. Activate Serratia gordonii S-2 on beef juice solid medium for three times, then pick a single colony and inoculate it in a 250mL Erlenmeyer flask containing 50mL beef juice liquid medium, 30°C, 200r / min Cultivate on a constant temperature shaker for 2 days to obtain a culture solution.

[0095] 2. After completing step 1, take the culture solution, centrifuge at 12000r / min for 1min, discard the supernatant, and collect the precipitate.

[0096] 3. After completing step 2, take the precipitate and resuspend it with 50 mL of sterile water to obtain a bacterial suspension (the concentration of Serratia grisneri S-2 is about 1.0×10 10 cfu / mL).

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Abstract

The invention discloses a strain of Serratia gaseri and its application. The Serratia grimesii provided by the present invention is Serratia grimesii S-2, and its preservation number in the General Microorganism Center of China Committee for Microorganism Culture Collection is CGMCC No. 13034. Experiments have proved that the Serratia gasseri S-2 provided by the present invention can inhibit Streptomyces scab and / or Streptomyces albicans, and has important application value for preventing and treating potato scab.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Serratia gordonii and its application. Background technique [0002] Potato scab is a plant disease that harms potato tubers. It is mainly manifested by the appearance of nearly round to amorphous suberized scab-like light brown lesions or plaques on the surface of tubers, and the texture is rough to the touch. Potato scab generally has two symptoms of reticulated lesions and cracked lesions. Usually, reticulated lesions and cracked lesions are limited to the cortex, but the quality and yield of the damaged potato pieces can still be reduced, and they are not resistant to storage, and the appearance of the diseased potato is unsightly, and the commodity grade is greatly reduced, resulting in certain economic losses. loss. In recent years, the area of ​​the disease has been increasing in Inner Mongolia, Hebei, Shandong and other regions, and the economic losses caused a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/425
CPCA01N63/10C12N1/20C12N1/205C12R2001/425
Inventor 张国青钞亚鹏仲乃琴赵盼杨敬
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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