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Mutein, method for producing said mutein, and gene encoding said mutein

A protein and gene technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, genetic engineering, etc.

Pending Publication Date: 2017-02-22
OSAKA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are also methods of increasing terpenoid production without genetic recombination, for example by using cholesterol biosynthesis inhibitors, but there is still room for improvement and further development of methods of increasing terpenoid production and their analogues The need for these technologies

Method used

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  • Mutein, method for producing said mutein, and gene encoding said mutein
  • Mutein, method for producing said mutein, and gene encoding said mutein
  • Mutein, method for producing said mutein, and gene encoding said mutein

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Embodiment

[0129] The present invention will be explained in detail with reference to examples, but the present invention is not limited to these examples.

[0130] (Suggestive evidence for the presence of new phosphorylation sites)

[0131] HMGR1 having a mutation in which serine 577 of Arabidopsis HMGR1 was replaced by alanine (hereinafter referred to as S577A-HMGR1) was transfected into an Arabidopsis mutant lacking endogenous HMGR1 to produce a mutant expressing S577A - Arabidopsis transformants of HMGR1 in which phosphorylation at serine 577 is known to reduce enzyme activity. ER fractions were collected from this transgenic plant expressing S577A-HMGR1 (HMGR is an ER-localized membrane protein). When the ER fraction containing S577A-HMGR1 was treated with phosphatase, its enzymatic activity was found to increase in the same manner as when the ER fraction containing wild-type HMGR from Arabidopsis expressing wild-type HMGR1 was treated with phosphorylated after enzyme treatment. ...

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Abstract

Provided is a mutein having mutations of specific amino acid residues of HMGR, which is a rate-limiting enzyme of isoprene monomer biosynthesis in the polyisoprenoid biosynthetic pathway. The invention relates to a mutein in which at least one amino acid residue selected from the group consisting of positions 91, 225, 257, 287, 339, 411, 470, 509, and 574 and amino acid residues of positions corresponding to these in Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-CoA reductase shown by SEQ ID NO:1 have been deleted or substituted by another amino acid residue in 3-hydroxy-3-methylglutaryl-CoA reductase.

Description

technical field [0001] The present invention relates to a mutated protein, a method for preparing the mutated protein, a gene encoding the mutated protein, a recombinant vector and a plant carrying the gene, control of mevalonate production and isoprenoid A method for ene production, and a method for controlling the activity of 3-hydroxy-3-methylglutaryl-CoA reductase. Background technique [0002] In the terpenoid biosynthetic pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (hereinafter also referred to as HMGR or HMG-CoA reductase) is an isoprene monomer (isopentenyl Diphosphate) is the rate-limiting enzyme in biosynthesis. It is known that the enzymatic activity of HMGR is controlled by reversible post-translational phosphorylation, in particular, inactivation of enzymatic activity by C-terminal serine phosphorylation is known to be a post-translational regulation mechanism of HMGR in animals. This has also been confirmed in plants, especially in Non-Patent Document 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/00A01H5/00C12N9/04C12N15/09C12P5/02C12P7/40
CPCC12N15/00C12N15/09C12P5/02C12P7/40C12N9/0006C12Y101/01034Y02A40/146A01N37/18C12N15/8243
Inventor 铃木启子R·杰科松村中俊哉高木启治井之上雪乃伏原和久
Owner OSAKA UNIV
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