Composite carrageenan enzyme and method for preparing selenized carrageenan oligosaccharides using the enzyme

A technology of compound carrageenan enzyme and selenized carrageenan, which is applied in the field of marine biology, can solve the problems of polluting the environment, difficulty in application, difficult control of reaction conditions of selenized carrageenan oligosaccharides, etc., and achieves the effect of mild reaction conditions and easy control

Active Publication Date: 2019-11-12
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reaction conditions for the preparation of selenized carrageenan oligosaccharides by this chemical method are not easy to control, pollute the environment, and the retention rate of the dialysis bag is low. The prepared selenized carrageenan oligosaccharides have low selenium content and are difficult to apply in industrial production.

Method used

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  • Composite carrageenan enzyme and method for preparing selenized carrageenan oligosaccharides using the enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation method of selenized carrageenan oligosaccharide comprises the following steps:

[0036] (1) Composition of compound carrageenase: 7 parts by weight of κ-carrageenase raw material, 2 parts by weight of ι-carrageenase raw material, and 1 weight part of λ-carrageenase raw material. Mix in a certain proportion in a sterile environment. The appearance is white powder; the total number of bacteria 5 The κ-type carrageenase of U, the enzyme activity is 1.4×10 5 The iota carrageenase and enzyme activity of U are 6.0×10 4 U lambda carrageenase.

[0037] (2) Hydrolysis of selenized carrageenan by compound carrageenan enzyme: prepare a selenized carrageenan solution with a mass fraction of 15% in distilled water, then add compound carrageenan enzyme solution according to the addition amount of 300U / ml, and react at 35°C for 9h Hydrolyze the selenized carrageenan, then heat and boil for 10 minutes to terminate the reaction, cool to room temperature, place at 60° ...

Embodiment 2

[0040] The preparation method of selenized carrageenan oligosaccharide comprises the following steps:

[0041] (1) The composition of the compound carrageenase: 8 parts by weight of κ-carrageenase raw material, 1 part by weight of ι-carrageenase raw material, and 1 weight part of λ-carrageenase raw material. Mix in a certain proportion in a sterile environment. The appearance is white powder; the total number of bacteria 5 The κ-type carrageenase of U, the enzyme activity is 6.9×10 4 The iota carrageenase and enzyme activity of U are 6.0×10 4 U lambda carrageenase.

[0042] (2) Utilize compound carrageenan enzyme to hydrolyze selenized carrageenan: prepare a selenized carrageenan solution with a mass fraction of 5% with distilled water, then add an appropriate amount of compound carrageenan enzyme solution according to the addition amount of 300U / ml, and heat it at 30°C After reacting for 9 hours, selenized carrageenan was hydrolyzed, then heated and boiled for 10 minutes t...

Embodiment 3

[0045] The preparation method of selenized carrageenan oligosaccharide comprises the following steps:

[0046] (1) The composition of the composite carrageenase: 6 parts by weight of κ-carrageenase raw materials, 2 parts by weight of ι-carrageenase raw materials, and 2 parts by weight of λ-carrageenase raw materials. Mix in a certain proportion in a sterile environment. The appearance is white powder; the total number of bacteria 5 The κ-type carrageenase of U, the enzyme activity is 1.4×10 5 The iota carrageenase and enzyme activity of U are 1.2×10 5 U lambda carrageenase.

[0047] (2) Utilize compound carrageenan enzyme to hydrolyze selenized carrageenan: prepare a selenized carrageenan solution with a mass fraction of 5% with distilled water, then add an appropriate amount of compound carrageenan enzyme solution according to the addition amount of 300U / ml, and heat it at 30°C After reacting for 9 hours, selenized carrageenan was hydrolyzed, then heated and boiled for 10 ...

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Abstract

The invention relates to composite carrageenase and a method for preparing selenium carrageenan oligosaccharide by the carrageenase. Each gram of the composite carrageenase comprises kappa carrageenase with enzyme activity of 2.1*10<5>-1.7*10<6>U, iota carrageenase with enzyme activity of 6.0*10<4>-4.8*10<5>U and lambda carrageenase with enzyme activity of 3.0*10<4>-2.4*10<5>U. The composite carrageenase has the function of synergistically degrading selenium carrageenan more sufficiently and uniformly, and has the advantages of mild reaction conditions, easiness in control, environmental protection, specificity, high efficiency and the like. The prepared oligosaccharide is more uniform in molecular weight, and the yield of the oligosaccharide is far higher than that of selenium carrageenan oligosaccharide prepared by a chemical method and a single enzyme method.

Description

technical field [0001] The invention relates to a compound carrageenan enzyme and a method for preparing selenized carrageenan oligosaccharides by using the enzyme, belonging to the technical field of marine organisms. Background technique [0002] At present, the understanding of selenium as an essential trace element in life is deepening day by day. Selenium is an essential trace element for the human body. It is known as the "King of Anti-cancer". Supplementing selenium can help improve the body's immunity and enhance physical fitness. At the same time, selenium is an extremely toxic element. Animals' nutritional requirements for selenium range from 0.1 to 0.3 ppm, and 2 to 10 ppm will cause chronic poisoning, while exceeding 10 ppm will cause acute poisoning and even sudden death. The content of selenium in the food chain of human society is decreasing, and the selenium deficiency diseases of animals and humans are increasing, and the trend of selenium deficiency is int...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/38C12P19/04A23K20/163A23K20/20A23K50/75C05D9/02
CPCC05D9/02C12N9/2468C12P19/04C12Y302/01083C12Y302/01157
Inventor 郑洲缪锦来
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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