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Explant acquisition method for Quercus acutissima Carruth tissue culture

A technology of tissue culture and acquisition method, which is applied in the direction of botany equipment and methods, culture medium, planting substrate, etc. It can solve the problems that the sprouts of big trees are difficult to meet the actual production and seedling needs, it is difficult to become a common material for tissue culture, and it is inconvenient to pick. , to achieve the effects of good quality explants, easy management of mother plants, convenient collection of shoots, and increased survival rate

Inactive Publication Date: 2017-03-22
LIUZHOU SANNONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the large trees are located in remote forest areas, which are inconvenient to pick, difficult to germinate, small in number and difficult to sterilize. Therefore, it is difficult for large tree sprouts to meet the actual production and seedling needs.
However, isolated embryos, sterile seedlings, cotyledons and needles are used as explant materials, and the speed and quantity of adventitious buds obtained are not as fast and as many as stem segments, so it is difficult to become common materials for tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Select the seeds of the excellent family of Quercus japonica with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 100-140 ° C. After natural cooling to room temperature, the seeds are sown in a 0.3% volume concentration. Sand bed disinfected with potassium permanganate solution.

[0026] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, sprayed with water and sprayed according to the situation, and apply a compound with a mass concentration of 0.5% every 7-10 days. Fertilizer solution, wherein the active ingredient of NPK in the compound fertilizer solution is more than or equal to 48%, and the sprouts will take root after 40 days.

[0027] When the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 40-60 cm, a ground diameter of 0.3-0.6 cm, a straight t...

Embodiment 2

[0030] Select the seeds of the excellent family of Quercus quercus with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 110°C. Potassium acid solution disinfected sand bed.

[0031] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, watered and sprayed according to the situation, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 8 days , wherein the active ingredient of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root after 40 days.

[0032] After the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 45-50 cm, a ground diameter of 0.3-0.4 cm, a straight trunk, and few side branches as the mother plant, and transplant them into a container cup with a size of 30 cm × 30 cm and a substrate. I...

Embodiment 3

[0035] Select the seeds of the excellent family of Quercus quercus with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 120 ° C. After natural cooling to room temperature, the seeds are sown in high manganese with a volume concentration of 0.3%. Potassium acid solution disinfected sand bed.

[0036] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15 cm × 15 cm, watered and sprayed according to the situation, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 9 days , wherein the active ingredient of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root after 40 days.

[0037] When transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 50-55 cm, a ground diameter of 0.4-0.5 cm, a straight trunk, and few side b...

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Abstract

The invention discloses an explant acquisition method for Quercus acutissima Carruth tissue culture, comprising processes as follows: seed selection and sowing, transplanting and management of bud seedlings, plantation and management of stock plant, and acquisition of explants. After two rounds of selecting excellent seed sources and excellent strong seedlings, plantation of stock plant is carried out, and proper sprout strips are cut off from stock plant and then disinfection and inoculation are carried out. The management of stock plant and acquisition of sprout strips are very convenient. The obtained explants has excellent quality, low contamination rate andmortality and high germination rate of adventitious buds, and sprouts are tidy. The method has strong operationality, meets requirements for establishment of a Quercus acutissima Carruth tissue culture intermediate propagation system, and has strong economic benefit, social benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and relates to a method for obtaining explants of oak tissue culture. Background technique [0002] Quercus acutissima Carruth (Quercus acutissima Carruth), also known as Qinggang and Oak Bowl Tree, is a tree of the genus Quercus in the family Fagaceae. It is 30 meters high and 1 meter in diameter at breast height. The bark is dark grayish brown and deeply longitudinally split. Born in the sunny slopes of mountains at an altitude of 60-2200 meters, it forms small pure forests or mixed forests. It is positive and likes light and humid climate. Cold-resistant, drought-resistant and barren, not resistant to water and humidity, not resistant to salt and alkali, it grows best on moist, fertile, deep, well-drained neutral to slightly acidic sandy loam, poor drainage or waterlogged land is not suitable for planting. Mixing with other tree species can form a good stem shape, deep ro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G17/00A01G9/10
CPCA01H4/00A01G17/005A01G24/00
Inventor 陈杏林
Owner LIUZHOU SANNONG TECH
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