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A novel wheat gene thmyc4e regulating anthocyanin synthesis and metabolism

An anthocyanin and gene technology, applied in genetic engineering, plant genetic improvement, angiosperms/flowering plants, etc., can solve problems such as plant phenotype loss

Active Publication Date: 2019-07-12
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Blockage of any of these reaction steps results in loss of plant phenotype

Method used

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  • A novel wheat gene thmyc4e regulating anthocyanin synthesis and metabolism
  • A novel wheat gene thmyc4e regulating anthocyanin synthesis and metabolism
  • A novel wheat gene thmyc4e regulating anthocyanin synthesis and metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 A novel wheat gene ThMYC4E that regulates anthocyanin synthesis and metabolism, the gene ThMYC4E has the nucleotide sequence shown in the first to 1707th positions of SEQ ID NO.1 in the sequence listing. It is screened from wheat varieties by specific primers ThMYC4EspF and ThMYC4EspR.

[0032] The protein encoded by the novel wheat gene ThMYC4E that regulates anthocyanin synthesis and metabolism has the amino acid sequence shown in the first to the 568th positions of SEQ ID NO.2 in the sequence list.

[0033] 1. Isolation of ThMYC4E gene

[0034] 1. Extraction of DNA and total RNA:

[0035] DNA was isolated from 1 g of 10-day-old seedlings (using Yan ZH, Wan YF, Liu KF, Zheng YL, WangDW, (2002) Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Methods in ScienceBulletin 47:220-225). Total RNA was extracted from the aleurone layer of ten blue seeds using Tiangen RNAprep Plant ...

Embodiment 2

[0099] Example 2 The new wheat gene ThMYC4E that regulates anthocyanin synthesis and metabolism is used as a recombinant vector, and the recombinant vector is used as a part of the host cell.

Embodiment 3

[0100] Example 3 The new wheat gene ThMYC4E that regulates anthocyanin synthesis and metabolism is used as a part of the host cell.

[0101] The host cells in the above Examples 2-3 refer to cells other than human or animal germ cells or embryonic stem cells.

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PUM

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Abstract

Provided is a wheat gene ThMYC4E for regulating anthocyanin synthesis. The sequence of the gene is represented by SEQ ID NO: 1 in a sequence table. ThMYC4E codes a transcription factor bHLH, and can be used for regulating the synthesis of anthocyanins in plants and the cultivation of transgenic plants.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a new wheat gene ThMYC4E that regulates anthocyanin synthesis and metabolism. Background technique [0002] The aleurone layer of blue-grain wheat contains four main types of anthocyanins (Abdel-Aal, E.S.M. and P.Hucl, Composition and stability of anthocyanins in blue-grained wheat. Journal of Agricultural and Food Chemistry, 2003. 51(8 ): p. 2174-2180). Anthocyanins endow plant organs and tissues with colorful colors, and have the function of attracting insects to help them pollinate (Joaquin-Cruz, E., et al., Anthocyanin and phenolic characterization, chemical composition and antioxidant activity of chagalapoli (Ardisia compressa K.) fruit: A tropicalsource of natural pigments. Food Research International, 2015. 70: p. 151-157), also has the function of helping plants develop specific disease resistance, protecting plants against biotic and abiotic stress factors, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
CPCC07K14/415C12N15/8243
Inventor 刘宝龙李娜张怀刚魏乐张波陈文杰刘登才
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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