Method for establishing fingerprint of rhizoma cimicifugae radix puerariae decoction composition and fingerprint

A technology of Shenma Gegen decoction and fingerprint spectrum, which is applied in important analysis fields, can solve problems such as no literature report on the composition of Shenma Gegen decoction, and achieve the effects of convenient operation, objective conclusion, and control of preparation quality.

Active Publication Date: 2017-03-22
CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Therefore, the technical problem to be solved in the present invention is to overcome the defects in the prior art that there is no fingerprint of the composition of Cimicifuga Radix and Radix Cimicifugae and establishment of the fingerprint of the composition of Cimicifuga Puerariae, thereby providing a Cimicifuga Method for establishing fingerprints of Pueraria decoction composition and its fingerprints

Method used

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  • Method for establishing fingerprint of rhizoma cimicifugae radix puerariae decoction composition and fingerprint
  • Method for establishing fingerprint of rhizoma cimicifugae radix puerariae decoction composition and fingerprint
  • Method for establishing fingerprint of rhizoma cimicifugae radix puerariae decoction composition and fingerprint

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Experimental program
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Effect test

Embodiment 1

[0078] Embodiment 1. prepare reference substance solution

[0079] Isoferulic acid reference substance solution Take an appropriate amount of isoferulic acid reference substance, accurately weigh it, put it in a brown measuring bottle, add 10% ethanol to make a solution containing 20 μg of isoferulic acid per 1 ml, and obtain it.

[0080] Cimicifume reference substance solution Take an appropriate amount of Cimicifume reference substance, accurately weigh it, put it in a brown measuring bottle, add 10% ethanol to make a solution containing 80 μg of isoferulic acid per 1 ml, and obtain it.

[0081] Paeoniflorin reference substance solution Take an appropriate amount of paeoniflorin reference substance, weigh it accurately, add methanol to make a solution containing 60 μg per 1 ml, and obtain it.

[0082] Paeonifloride reference substance solution Take an appropriate amount of paeonifloride reference substance, accurately weigh it, put it in a brown measuring bottle, add 10% e...

Embodiment 2

[0089] Embodiment 2. Preparation of Radix Cimicifugae Radix Decoction Composition

[0090]The first Cimicifuga Gegen Decoction composition: Weigh the four-flavored decoction pieces with the following weight ratio: 300g Cimicifuga; 9g (2g Cimicifuga, 3g Pueraria Radix, 2g Radix Paeoniae Alba, 2g Zhilicorice) are placed in a 2L decoction casserole, add 300ml water, soak for 30 minutes, cover and cook, heat (voltage value about 220V) to boiling (about 10 Minutes), keep boiling slightly (voltage value about 170V) to about 100ml of medicinal liquid (about 38 minutes), use two layers of medical gauze to filter out medicinal residues, and obtain.

[0091] The second Cimicifuga Gegen Decoction composition: Weigh the following decoction pieces Cimicifuga 666.7g, Pueraria 1000g, Radix Paeoniae Alba 666.7g, Radix Glycyrrhizae 666.7g by weight, cut into coarse particles with a particle size of 2mm-8mm, add 12 times the amount of water Water, decocted for 30 minutes, filtered to obtain ...

Embodiment 3

[0092] Embodiment 3: the selection of chromatographic conditions

[0093] (1) Selection of detection wavelength

[0094] Octadecylsilane-bonded silica gel is used as filler: methanol is used as mobile phase A, 0.1mol / L phosphoric acid is used as mobile phase B, and gradient elution is carried out according to the following table. The injection volume is 10 μl, and the flow rate is 1.0ml / min , Column temperature: 30°C. The gradient program is as follows.

[0095] Table 1 Gradient elution program 1

[0096]

[0097] Using HPLC multi-wavelength detection technology, the detection wavelengths are λ=316, λ=290, λ=270, λ=250, λ=237, λ=230, and are evaluated by the number of chromatographic peaks and resolution. The results are shown in figure 1 ,2.

[0098] From the 3D spectral scanning graph, it can be known that most of the substances contained in Cimicifuga Gegen Decoction are at the terminal absorption (210nm-250nm). figure 1 From the wavelengths investigated, it can b...

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Abstract

The invention discloses a method for controlling the quality of a rhizoma cimicifugae radix puerariae decoction composition, and belongs to the field of traditional Chinese medicine analysis. The method adopts a high performance liquid chromatographic method for establishing a fingerprint, chromatographic conditions are as follows: an octadecyl silane bonded silica gel chromatography column is used as a chromatography column, flow rate is 0.9ml / min-1.1ml / min, column temperature is 25 to 35 DEG, an UV detector is used as a detection instrument, detection wavelength is 210nm-250nm; theoretical plate number is not less than 4000 on the basis of a puerarin peak; a reference solution is a methanol solution of daidzin; an acetonitrile-0.1% phosphoric acid solution system is used as a moving phase, and gradient elution is performed in the following order shown in the specification. The fingerprint fully reflects the quality information of a rhizoma cimicifugae radix puerariae decoction, so that the purpose of more effective and comprehensive control of the quality of the rhizoma cimicifugae radix puerariae decoction product can be achieved.

Description

technical field [0001] The invention belongs to the important analysis field, and in particular relates to the fingerprint of the composition of Cimicifuga Radix and Gegen Decoction. Background technique [0002] The composition of Cimicifuga Gegen Decoction is composed of four traditional Chinese medicines of Radix Cimicifugae, Pueraria Radix Radix Radix Radix Radix Radix Radix Puerariae, Radix Paeoniae Alba, Radix Paeoniae Alba, Radix Glycyrrhizae. Dysentery, hepatitis and other diseases. In addition, this prescription is used by children, and it is very effective for the elderly and children with cold, high fever and chills. In order to more comprehensively and effectively control the quality control level of this traditional Chinese medicine composition, and to be in line with international standards, so as to ensure the safety and efficacy of clinical medication, it is necessary to adopt more advanced quality control methods for this traditional Chinese medicine composi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈世彬覃旭陈周全刘志刚谭沛马鹏岗姚少姿刘志东
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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