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Rauvolfia verticillate rooting culture medium and application thereof

A rooting medium and Rauwolfia root technology are applied in application, plant regeneration, horticulture, etc., which can solve the problems of slow growth rate, long cycle and low survival rate, and achieve the effect of promoting rooting rate and saving resources.

Inactive Publication Date: 2017-04-26
叶宗耀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The propagation of Rauwolfia is mainly seed and cutting propagation, but the germination rate of seed propagation is low, the growth rate is slow, the cycle is long and other shortcomings, while the cutting propagation operation is complicated, the survival rate is low and other shortcomings cannot be widely promoted. Therefore, in order to promote Rauwolfia The rapid propagation of Rauvolwolfia has been carried out on the culture medium of Rauwolfia tissue, which can get rid of the influence of external environment on the plants, and can screen the excellent varieties and optimize the varieties. reproduction is very important

Method used

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  • Rauvolfia verticillate rooting culture medium and application thereof
  • Rauvolfia verticillate rooting culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of Rauwolfia rooting medium, described rooting medium is 1 / 2MS medium+0.15mg / L N-phenyl-N'-1,2,3-thiadiazole-5-urea+0.6mg / L 2,4-dichlorophenoxyacetic acid+850mg / L Rauwolfia root powder.

[0026] The preparation method of the Rauwolfia root powder is as follows: crush the Rauwolfia root to a particle size of 0.5 cm, vacuum-dry at a temperature of 28° C. for 48 hours, and then grind to 100 mesh.

[0027] The preparation method of the described Rauwolfia rooting medium: N-phenyl-N'-1,2,3-thiadiazole-5-urea, 2,4-dichlorophenoxyacetic acid and Rauwolfia root powder , added to the diluted MS medium, stirred evenly, adjusted the pH to 5.8 with 1M hydrochloric acid solution, and sterilized at 120°C for 25 minutes to obtain.

Embodiment 2

[0029] A kind of Rauwolfia rooting medium, described rooting medium is 1 / 2MS medium+0.1mg / L N-phenyl-N'-1,2,3-thiadiazole-5-urea+0.3mg / L 2,4-dichlorophenoxyacetic acid+1200mg / L Rauwolfia root powder.

[0030] The preparation method of described rauwolfia root powder is similar to Example 1.

[0031] The preparation method of described Rauwolfia rooting medium is similar to Example 1.

Embodiment 3

[0033] A kind of Rauwolfia rooting medium, described rooting medium is 1 / 2MS medium+0.2mg / L N-phenyl-N'-1,2,3-thiadiazole-5-urea+0.8mg / L2,4-dichlorophenoxyacetic acid+500mg / L Rauwolfia root powder.

[0034] The preparation method of the described Rauwolfia root powder is similar to Example 1.

[0035] The preparation method of described Rauwolfia rooting medium is similar to Example 1.

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Abstract

The invention belongs to the technical field of rooting of tissute culture, and particularly relates to a rauvolfia verticillate rooting culture medium and application thereof. The rooting culture medium is prepared from, 1 / 2 a MS culture medium, 0.1-0.2 mg / L N-Phenyl-N'-(1,2,3-thiadiazol-5-yl)urea, 0.3-0.8 mg / L 2,4-dichlorophenoxyacetic acid and 500-1200 mg / L of rauvolfia verticillate root powder. The survival rate of rauvolfia verticillate seedlings cultivated by the rauvolfia verticillate rooting culture medium is 95% or above, strong seedlings can be obtained, the rooting speed is high, the roots are developed, the quality of the seedlings is good, components of the culture medium are wide in source, the culture speed is high, and the rauvolfia verticillate rooting culture medium is suitable for large-area planting.

Description

technical field [0001] The invention belongs to the field of plant regeneration through tissue culture technology, and specifically relates to a rauwolfia rooting medium and its application. Background technique [0002] Rauwolfia: An upright evergreen shrub, up to 3m high, with latex, which belongs to a kind of medicinal material. Its main functions are sedation, lowering blood pressure, promoting blood circulation and relieving pain, clearing heat and detoxifying. For hypertension, headache, dizziness, insomnia, persistent high fever, external use for bruises, snake bites, etc. Reserpine, amoline, rolaidine, serpentine, amorphine, beine, rauwolfin, trylosamine, piranthine, a Various alkaloids such as monoterpene alkaloids, the content is 1-2%, wherein reserpine has good antihypertensive effect. Rauwolfia still contains 0.2% ursolic acid, which has a wide range of anti-tumor effects and is very likely to become a low-toxic and effective new anti-cancer drug. Therefore, R...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 叶宗耀
Owner 叶宗耀
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