Application of Bacillus subtilis in degrading polyphenols in tobacco products

A technology for Bacillus subtilis and tobacco products, which is applied to the application field of Bacillus subtilis xp strain in degrading polyphenols in tobacco products, and can solve the problems of easy mildewing of tobacco leaves, overstocked tobacco leaves in inventory, long alcoholization period, and the like, Achieve good application value and application prospects, reduce the content of polyphenols, and improve the effect of tobacco grade

Active Publication Date: 2018-03-27
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificial aging is fast and the cycle is short, but the aging effect is poor; natural aging is to stack the tobacco leaves in the storage warehouse according to certain requirements, and carry out the aging under natural conditions. The time is generally 1 to 3 years
Although the effect of natural aging is better, there are still some disadvantages, such as: 1) During the aging process, the tobacco leaves are prone to breeding insects; 2) Due to changes in temperature and humidity, the tobacco leaves are prone to mildew; 3) The aging cycle is long, which is easy to cause The backlog of tobacco leaves in stock and the occupation of a large amount of funds

Method used

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  • Application of Bacillus subtilis in degrading polyphenols in tobacco products
  • Application of Bacillus subtilis in degrading polyphenols in tobacco products
  • Application of Bacillus subtilis in degrading polyphenols in tobacco products

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Embodiment

[0024] In order to use the Bacillus subtilis xp strain to degrade polyphenols in tobacco products, it is first necessary to properly amplify the strain and prepare it to contain a certain amount of active bacterial agent before it can be applied. The specific preparation method of the bacterial agent is as follows .

[0025] (1) Strain activation, using an inoculation loop to pick up the strains on the slant medium (the formula is the same as that of LB liquid medium, only add 15g / L agar powder), place them in LB liquid medium for suspension culture, and incubate at 30°C for 12 hours Activation of bacteria;

[0026] (2) Strain expansion, transfer the bacterial liquid after the activation of the strain in step (1) to LB liquid culture medium, and cultivate it on a shaker at 35°C until OD 600 =3.5 or so, it can be used as bacterial agent for degrading tobacco products after being evenly suspended.

[0027] When the above bacterial agent is specifically used to degrade polyphen...

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Abstract

The invention belongs to the technical field of tobacco preparation, and specifically relates to the application of a bacillus subtilis xp strain in degrading polyphenols in tobacco products. The Bacillus subtilis is a Bacillus subtilis xp strain, which is now preserved in the China Center for Type Culture Collection, with a preservation number of CCTCC No: M 2011452; the strain is used to reduce the content of polyphenols in tobacco products; when applied, the The Bacillus subtilis xp strain was prepared as an inoculum, which was evenly sprayed on the surface of tobacco products at a mass ratio of 1.0% of tobacco products, and then stored and alcoholized for 30-40 days at 30°C and 55-65% humidity. Through the selection of specific Bacillus subtilis strains, the present invention proves that it can effectively reduce the content of polyphenols in tobacco products, improve the grade of tobacco, and accelerate the aging process of tobacco leaves while ensuring the aging effect.

Description

technical field [0001] The invention belongs to the technical field of tobacco preparation, and specifically relates to the application of a bacillus subtilis xp strain in degrading polyphenols in tobacco products. Background technique [0002] Bacillus subtilis is a kind of Bacillus genus, which is widely distributed in soil and decayed organic matter, and is easy to reproduce in subtilis juice. The surface of its colony is rough and opaque, stained white or yellowish, and can use protein, various sugars and starch to decompose tryptophan to form indole, which is widely used in genetic research. In addition, Bacillus subtilis itself can synthesize enzymes such as α-amylase, neutral protease, lipase, cellulase, etc., and has good application prospects in degrading complex macromolecular compounds. Therefore, Bacillus subtilis has gradually become a A hotspot of attention and research. [0003] Tobacco leaf fermentation is also called tobacco leaf alcoholization. Its essenc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/125
CPCA24B15/20
Inventor 杨宗灿张展马宇平陈芝飞于建春彭玉富周浩郝辉聂聪
Owner CHINA TOBACCO HENAN IND
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