Preparation method of collagen-hydroxyapatite stent containing nanometer silver

Abstract

The invention relates to a preparation method of a collagen-hydroxyapatite stent containing nanometer silver. The nanometer silver is prepared through a liquid phase reduction reaction; the nanometer silver and a collagen-hydroxyapatite solution are uniformly mixed, and are then subjected to one-step freeze drying and crosslinking to prepare and obtain the collagen-hydroxyapatite stent containing nanometer silver, wherein the concentration range of the nanometer silver is 10 ug / ml to 50ug / ml; and the particle diameter of the nanometer silver is 10 to 30nm. The stent provided by the invention has the advantages that required materials are simple; no complicated technical requirements and no high-end equipment requirements are needed; the cost is low; the popularization is easy; a reasonable nanometer silver slow-release system is provided for a wound surface; the long-time effective medicine slow release is provided for the wound surface; and the medicine concentration on the wound surface keeps balanced, effective and non-superfluous.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of a collagen-hydroxyapatite scaffold containing nano-silver. Background technique [0002] Hydroxyapatite and collagen are the most important inorganic and organic components in natural bone, and both have good biological properties, but they have different defects when used alone, and it is difficult to meet the performance requirements of tissue engineering for biomaterials. The combination of collagen and hydroxyapatite makes better use of the cohesiveness of collagen and overcomes the fluidity of hydroxyapatite particles. [0003] Nano-silver has excellent heat transfer, electrical conductivity, surface activity and catalytic properties, and has great application value in the fields of optics, catalysis, microelectronics, biosensing, and antibacterial. Nano-silver powders with different shapes have different properties. For example, sph...

AI Technical Summary

Problems solved by technology

[0004] Due to the high biological activity of nano silver, when it is applied to tissue engineering, it will be released into the wound or the target organ, and it will be preferentially captured by the related proteins, chloride ions and groups in the wound exudate or tissue, resulting in the formation of silver nanoparticles in tissue engineering. Abnormal accumulation of high concentrations in some parts, although it can effectively participate in antibacterial, these excess nano silver will accumulate in epidermal cells, macrophages, fibroblasts, leading to cell apoptosis, and eventually lead to non-healing or delayed healing of the wound

In general, unrestrained use or uncontrolled release of nano-silver is bound to be counterproductive and not beneficial to wound repair