Preparation method of collagen-hydroxyapatite stent containing nanometer silver

A technology of hydroxyapatite and nano-silver sol, which is applied in nanotechnology, medical science, prosthesis, etc., can solve the problems of no benefit in wound repair, wound non-healing, delayed healing, etc., and achieves easy promotion, simple materials, and production short cycle effect

Inactive Publication Date: 2017-04-26
王淑芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the high biological activity of nano silver, when it is applied to tissue engineering, it will be released into the wound or the target organ, and it will be preferentially captured by the related proteins, chloride ions and groups in the wound exudate or tissue, resulting in the formation of silver nanoparticles in tissue engineering. Abnormal accumulation of high concentrations in some parts, although it can effectively participate in antibacterial, these excess nano silver will accumulate in epidermal cells, macrophages, fibroblasts, leading to cell apoptosis, and eventually lead to non-healing or delayed healing of the wound
In general, unrestrained use or uncontrolled release of nano-silver is bound to be counterproductive and not beneficial to wound repair

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of a collagen-hydroxyapatite scaffold containing nano-silver is achieved through the following processes:

[0019] (1) adopting silver nitrate and potassium borohydride and adding maleic anhydride to carry out liquid phase reduction reaction to prepare nano-silver sol; adding maleic anhydride is 3% of silver nitrate quality; reaction temperature is 40 DEG C;

[0020] (2) Take the nano-silver sol and stir it for 10 minutes, heat it with microwave under airtight conditions (select Beijing Xianghu HX-300A computer microwave ultrasonic combined synthesizer as the microwave source), first react at 300W microwave power for 2.25min, and then react at 120W microwave React under power for 2.75min to obtain nano-silver solution;

[0021] (3) Dissolve type Ⅰ collagen and hydroxyapatite derived from bovine Achilles tendon in 0.5mol / L acetic acid solution at a mass ratio of 9:1, add a certain amount to the nano-silver solution with a target concentration of 10pp...

Embodiment 2

[0023] A preparation method of a collagen-hydroxyapatite scaffold containing nano-silver is achieved through the following processes:

[0024] (1) adopting silver nitrate and potassium borohydride and adding maleic anhydride to carry out liquid phase reduction reaction to prepare nano-silver sol; adding maleic anhydride is 3% of silver nitrate quality; reaction temperature is 30 DEG C;

[0025] (2) Take the nano-silver sol and stir it for 10 minutes, heat it with microwave under airtight conditions (select Beijing Xianghu HX-300A computer microwave ultrasonic combined synthesizer as the microwave source), first react at 250W microwave power for 2.5min, and then in 150W microwave Reaction under power for 2.5min to obtain nano-silver solution;

[0026] (3) Dissolve type Ⅰ collagen and hydroxyapatite derived from bovine Achilles tendon in 0.5mol / L acetic acid solution at a mass ratio of 9:1, add a certain amount to the nano-silver solution with a target concentration of 10ppm, an...

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Abstract

The invention relates to a preparation method of a collagen-hydroxyapatite stent containing nanometer silver. The nanometer silver is prepared through a liquid phase reduction reaction; the nanometer silver and a collagen-hydroxyapatite solution are uniformly mixed, and are then subjected to one-step freeze drying and crosslinking to prepare and obtain the collagen-hydroxyapatite stent containing nanometer silver, wherein the concentration range of the nanometer silver is 10 ug / ml to 50ug / ml; and the particle diameter of the nanometer silver is 10 to 30nm. The stent provided by the invention has the advantages that required materials are simple; no complicated technical requirements and no high-end equipment requirements are needed; the cost is low; the popularization is easy; a reasonable nanometer silver slow-release system is provided for a wound surface; the long-time effective medicine slow release is provided for the wound surface; and the medicine concentration on the wound surface keeps balanced, effective and non-superfluous.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of a collagen-hydroxyapatite scaffold containing nano-silver. Background technique [0002] Hydroxyapatite and collagen are the most important inorganic and organic components in natural bone, and both have good biological properties, but they have different defects when used alone, and it is difficult to meet the performance requirements of tissue engineering for biomaterials. The combination of collagen and hydroxyapatite makes better use of the cohesiveness of collagen and overcomes the fluidity of hydroxyapatite particles. [0003] Nano-silver has excellent heat transfer, electrical conductivity, surface activity and catalytic properties, and has great application value in the fields of optics, catalysis, microelectronics, biosensing, and antibacterial. Nano-silver powders with different shapes have different properties. For example, sph...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/54A61L27/44A61L27/56B22F9/24B82Y40/00
CPCA61L27/46A61L27/446A61L27/54A61L27/56A61L2300/104A61L2300/404A61L2300/602B22F9/24B82Y40/00
Inventor 王淑芳
Owner 王淑芳
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