Targeting antibacterial peptide and preparation method and application thereof

Abstract

The invention discloses a targeting antimicrobial peptide and a preparation method and application thereof. A sequence of the targeting antimicrobial peptide cCF10-C4 is shown in a sequence table SEQ ID No.1. The preparation method comprises the steps that enterococcus faecalis serves as a target bacterium to obtain the targeting antimicrobial peptide cCF10-C4 through design, a series of multi-structural-domain antimicrobial peptide molecules having selectivity to the enterococcus faecalis are established in a heterozygous mode. The design of a series of antimicrobial peptide molecules mainly includes two independent functional areas, namely a sterilization area and an identification area respectively. The targeting antimicrobial peptide has targeting selection capability to the target bacterium. The targeting antimicrobial peptide cCF10-C4 is conductive to reestablishment of microecological balance after treatment.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a targeted antibacterial peptide and its preparation method and application. Background technique

[0002] Antimicrobial peptides are a class of small molecule polypeptides that exist in the natural immune defense system of organisms, participate in the immune defense function of the body, and are the first barrier for the body to resist the invasion of pathogenic microorganisms. Antimicrobial peptides are usually composed of 12-50 amino acid residues, with a molecular weight of less than 10kDa. Their common feature is that they contain positively charged amino acids and hydrophobic amino acids, and are amphipathic and cationic. Insects and even cancer cells have killing or inhibiting effect.

[0003] However, antimicrobial peptides, like traditional antibiotics, are mostly broad-spectrum antibacterial drugs, which not only exert great selective pressure on microbial res...

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