Simple and low cost method for detecting activity of alkaline phosphatase
A phosphatase activity and low-cost technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problems of bulky, high analysis costs, and expensive instruments. , to achieve broad application prospects, reduce analysis costs, and make the analysis process simple and easy
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Embodiment 1
[0019] Use a simple and low-cost alkaline phosphatase activity detection method to detect 5 U / mL alkaline phosphatase sample solution and blank sample (buffer solution without analyte).
[0020] Such as figure 1 As shown, the specific implementation process is as follows: Step 1, add 15 µL 5 U / mL alkaline phosphatase sample solution (prepared by 10 mM tris-HCl buffer solution containing 100 mM NaCl, pH7. 4), followed by adding 15 µL of 200 µM pyrophosphate solution (prepared from 10 mM tris-HCl buffer solution containing 100 mM NaCl, pH 7.4) to mix with it, and reacting at 37 °C for 60 min, then adding 15 µL of 50 µM copper nitrate aqueous solution, react at room temperature for 15 min, then continue to add 15 µL 1 mg / mL glucoamylase solution (prepared from 20 mM acetic acid-sodium acetate buffer solution, pH 4.5), react at room temperature for 70 min, then add 15 µL 2 % soluble starch solution (prepared by heating ultrapure water with a resistivity of 18.2 MΩ cm), and reacte...
Embodiment 2
[0023] A simple and low-cost alkaline phosphatase activity detection method was used to detect the alkaline phosphatase sample solution with an activity concentration ranging from 0.0375 to 5 U / mL.
[0024] Such as figure 1 As shown, the specific steps for detecting each alkaline phosphatase sample solution in the present embodiment are: Step 1, add 15 μL of an alkaline phosphatase sample solution of a certain activity concentration (contained by 100 mM NaCl in 10 mM tris-HCl buffer solution, pH 7.4), then add 15 µL of 200 µM pyrophosphate solution (prepared from 100 mM NaCl in 10 mM tris-HCl buffer solution, pH 7.4) to mix with it, and place React at 37 °C for 60 min, continue to add 15 µL of 50 µM copper nitrate aqueous solution, react at room temperature for 15 min, and then continue to add 15 µL of 1 mg / mL glucoamylase solution (prepared from 20 mM acetic acid-sodium acetate buffer solution, pH 4.5 ), reacted at room temperature for 70 min, then added 15 µL of 2% soluble ...
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