Streptomyces griseorubens pectinesterase as well as coding gene and application thereof

A technology of pectin esterase coding and Streptomyces grisea, which is applied in the fields of genetic engineering and modern enzyme engineering, can solve the problems of few examples, achieve strong tolerance, high-efficiency expression, and improve stability
CN106636035AInactive Publication Date: 2017-05-10SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2017-05-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses streptomyces griseorubens pectinesterase as well as a coding gene and an application thereof. The pectinesterase gene is cloned from streptomyces griseorubens; a nucleotide sequence of the gene is shown as SEQ ID NO.1 or is shown as SEQ ID NO.2 after codon optimization; an amino acid sequence of the coded pectinesterase is shown as SEQ ID NO.3; and the invention also discloses applications of the pectinesterase and the coding gene thereof in degrading pectin. According to the invention, by adding a soluble label to target protein and through peripheral space expression of the target protein, the bio-activity of the protein is guaranteed, and meanwhile, subsequent protein purification is facilitated. The pectinesterase is alkaline and high-temperature resistant, and the enzyme (pectinesterase) has a quite strong resisting capacity to a plurality of metal ions and enzymatic activity inhibitors; therefore, the enzyme has great significance in practical production and application.
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Description

technical field

[0001] The invention relates to the technical fields of genetic engineering and modern enzyme engineering, and relates to a pectin esterase of Streptomyces grisea and its encoding gene and its application, in particular to a pectin esterase and its encoding gene sequence, including the encoding gene sequence The recombinant expression vector, the transgenic recombinant bacteria and the application of the pectin esterase. Background technique

[0002] Pectinase is widely present in fruits and various microorganisms, and is a general term for a class of complex enzymes composed of many single enzymes that can act on pectin. Pectinase has main application significance in the fields of ramie degumming, wood preservation and alcohol fermentation, and has been widely used in the food industry.

[0003] Pectin esterase (EC 3.1.1.11) is an enzyme that catalyzes and hydrolyzes pectin to produce pectic acid and methanol. Pectinase has high specificity to polygalactur...

Claims

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