Real-time fluorescent quantitative PCR (polymerase chain reaction) identification reagent kit and identification method for bird's nest products

Abstract

The invention discloses a real-time fluorescent quantitative PCR (polymerase chain reaction) identification reagent kit and an identification method for bird's nest products. The real-time fluorescent quantitative PCR identification reagent kit for the bird's nest products comprises forward primers with sequences SEQ ID NO.1, reverse primers with sequences SEQ ID NO.2 and TaqMan fluorescent probes with sequences SEQ ID NO.3; fluorescent reporter groups can be labeled by 5' ends of the TaqMan fluorescent probes, and non-fluorescent quencher groups can be labeled by 3' ends of the TaqMan fluorescent probes. The real-time fluorescent quantitative PCR identification reagent kit and the real-time fluorescent quantitative PCR identification method for the bird's nest products have the advantages that the real-time fluorescent quantitative PCR identification method is intuitive and highly specific, is high in speed during detection, includes simple steps, is good in repeatability and high in sensitivity and is extremely high in operability when used for identifying the bird's nest products, and the bird's nest products can be repeatedly quantified.

Description

technical field [0001] The invention relates to an identification method for bird's nest products, in particular to a real-time fluorescence quantitative PCR identification method for bird's nest products and a related kit. Background technique [0002] The bird's nest is a nest built by several birds of the Swift family (Apodidae) and the genus Collocalia during the breeding period by mixing the secreted saliva or gastric juice with their own feathers, weeds, moss, etc., and condenses in sparsely populated areas. among cliffs or caves. Bird's nest, also known as jelly, was first recorded in the book "Compendium of Materia Medica" as a precious medicinal material suitable for medicine and diet. Modern clinical trials have confirmed that bird's nest has certain medicinal value. It is not only an effective "rare medicine" for preventing influenza virus, but also a tonic with high nutritional value. Rare things are more expensive, so they have the reputation of "Oriental Treas...

AI Technical Summary

Problems solved by technology

[0003] At present, the identification methods of bird's nest mainly include physical and chemical identification, spectral identification, chromatographic identification, biomolecular identification, sensory identification and a combination of multiple methods. These methods have the characteristics of low accuracy, low sensitivity, and poor reproducibility.

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