A Gene Codon Optimization Method Based on Codon Pair Usage Frequency

Abstract

The invention provides a method for optimizing genetic codons based on the codon-pair usage frequency. A target gene is optimized by means of the codon-pair usage frequency, a single condon usage frequency is considered, the situation of the combined use of two codons, namely, the relationship of adjacent codons, is also considered at the same time, the selection of a codon with a higher frequency when two tRNAs in species are used together plays a key role in optimizing the target gene.

Description

technical field [0001] The invention relates to a gene codon optimization method based on codon pair usage frequency. Background technique [0002] In order to increase the expression of gene products, gene code optimization is a common means of gene expression regulation in molecular biology, and codon optimization plays a very critical role in gene expression regulation. A common method is to analyze the codon usage frequency of a certain species, and select the codon with high frequency as the candidate codon for optimizing the target gene. [0003] "Codon pair" is composed of two adjacent codons in orf, because during protein translation, the A and P positions of the ribosome must combine with two tRNAs at the same time, and then add amino acids to the synthesized polypeptide one by one, so that Peptide extension. However, the spatial structure of the tRNA bound to the ribosome determines the difficulty of its entry. If two tRNAs bind to the ribosome and conflict with ...

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Problems solved by technology

However, the spatial structure of the tRNA bound to the ribosome at the same time determines the difficulty of its entry. If two tRNAs bind to the ribosome and conflict with each other, it will be difficult to bind and enter, then protein synthesis will be hindered or the synthesis speed will be slowed down.

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