Extraction method of anthocyanin

An extraction method and anthocyanin technology, applied in the direction of organic chemistry, can solve the problems of poor product quality and low stability, and achieve the effects of preventing oxidation, product safety and stability, and improving extraction effect.

Inactive Publication Date: 2017-05-17
临海市利科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the solvent method for extracting anthocyanins contains more impurities such as sugars, organic acids, and proteins during the extraction process, resulting in poor product quality and low stability. To o

Method used

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  • Extraction method of anthocyanin
  • Extraction method of anthocyanin
  • Extraction method of anthocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 100Kg of the original fruit of Indigo indigo, add 3Kg of sulfur dioxide, add 5Kg of 30% hydrochloric acid, pulverize, ferment naturally, add 1Kg of cellulase, 1Kg of amylase, 1Kg of lipase for enzymolysis, and filter to obtain the enzymatic hydrolyzate of Indigo indigo. Slowly add the indigo enzymatic hydrolyzate to the D-101 macroporous resin column, wash with 60% ethanol solution, and concentrate the washing liquid under reduced pressure until there is no ethanol smell, adjust the pH to 9, centrifuge, and take the clear The liquid is the indigo fruit extract. Use deionized water to adjust the mass concentration of anthocyanins in the indigo fruit extract to 1.2 mg / mL.

Embodiment 2

[0031] Take 100Kg of the original fruit of Cedarwood, add 1Kg of sulfur dioxide, add 10Kg of 30% hydrochloric acid, pulverize it, ferment naturally, add 5Kg of compound pectinase for enzymolysis, and filter to obtain the enzymatic hydrolyzate of Cedarwood. Slowly add the enzymatic hydrolyzate of edulis to AB-8 macroporous resin column, wash with 90% ethanol solution, and concentrate the washing liquid under reduced pressure until there is no ethanol smell, adjust the pH to 9, centrifuge, and take the clear The liquid is the indigo fruit extract. Use deionized water to adjust the mass concentration of anthocyanins in the indigo fruit extract to 1.2 mg / mL.

Embodiment 3

[0033] Take 100Kg of the original fruit of L. indigo, add 5Kg of sulfur dioxide, add 1Kg of 30% hydrochloric acid, pulverize, ferment naturally, add 5Kg of compound pectinase for enzymolysis, and filter to obtain the enzymatic hydrolyzate of L. indigo. Slowly add the enzymatic hydrolyzed solution of edulis to DM301 macroporous resin column, wash with 20% ethanol solution, and concentrate the washing liquid under reduced pressure until there is no ethanol smell, adjust the pH to 9, centrifuge, and take the clear liquid Indigo fruit extract. Use deionized water to adjust the mass concentration of anthocyanins in the indigo fruit extract to 1.2 mg / mL.

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Abstract

The invention discloses an extraction method of anthocyanin. The method comprises the following steps: (1) putting original Lonicera caerulea fruits into a container, introducing sulfur dioxide, adding 30% hydrochloric acid, pulverizing, fermenting, carrying out enzymolysis, and filtering to obtain a Lonicera caerulea enzymolysis solution; (2) dropwisely adding the Lonicera caerulea enzymolysis solution into a macroporous resin column, washing with a 10-90% ethanol solution, concentrating the washing solution under reduced pressure until no ethanol smell emits, regulating the pH value to 9, and carrying out centrifugal separation to obtain a Lonicera caerulea extracting solution; and (3) adding the Lonicera caerulea extracting solution and an amide ionic liquid disclosed as Formula (I) into a reaction vessel, extracting with stirring at 5-50 DEG C for 1-50 hours, standing to stratify, separating out the organic phase, and determining the content. In Formula (I), R<1> and R<2> are respectively C1-C10 amido or C1-C10 alkyl; and L<-> is BF4<->, PF6<->, OAC<->, CF3SO3<-> or N(SO2CF3)2<->.

Description

technical field [0001] The invention relates to a method for extracting anthocyanins, in particular to a method for separating and extracting anthocyanins from blueberry extracts. Background technique [0002] Anthocyanins are flavonoids, which are natural water-soluble pigments widely present in plants. Because they mainly exist in the form of glycosides, they are also called anthocyanins. They are the most effective natural water-soluble free radical scavengers found today. Anthocyanins can delay aging, activate macrophages, protect serum immunoglobulins from free radicals, improve immunity, reduce the incidence of cardiovascular and cerebrovascular diseases, reduce the incidence of diabetes and its complications, and improve degenerative Alzheimer's disease, protect the integrity of genes, reduce the incidence of cancer, anti-inflammation, prevent vision loss and many other effects. [0003] Blueberries are rich in anthocyanins, and there are a lot of natural blueberries...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 裴文何甫长刘乘风洪秋玲朱鹏超卢玉杰马光明
Owner 临海市利科生物科技有限公司
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