Medicament for accelerated wound healing
A wound healing and drug technology, applied in the direction of drug combination, drug delivery, active ingredients of hydroxyl compounds, etc., can solve the problems of scarcity of natural sandalwood oil and expensive raw materials
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[0128] Characterization of OR2AT4 in a heterologous expression system
[0129] In order to define the function of a receptor in tissue, it is important to understand the ligand to ensure the ability of the receptor to be activated in the native system. Most ORs have not been de-orphaned so far. Therefore OR2AT4 identified in human keratinocytes was first characterized more accurately in a heterologous expression system and the receptor domain was determined.
[0130] Heterologous expression of OR2AT4 in Hana3A‐cells
[0131] Hana3A-cells stably expressing endogenous olfactory transduction factors were used as a heterologous expression system, which targetedly assist OR to insert into the cell membrane and improve its activity. In addition, the addition of the first N-terminal amino acid at position 20 of the rhodopsin (Rho-tag) to the N-terminus of the OR coding sequence was also used to facilitate heterologous expression.
[0132] To detect heterologous expression of OR2AT...
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