Marine microcosm system and its construction method and method for rapidly assessing the biological toxicity of oil spill pollutants in the ocean
A technology of biological toxicity and pollutants, applied in the fields of botany equipment and methods, plant cultivation, seaweed cultivation, etc., can solve the problem of inability to accurately assess the impact of oil and other pollutants on marine biological systems, and inability to restore the real environment and biological composition of the surface sea area, etc. problem, to achieve the effect of fast and accurate evaluation method and simple construction method
Inactive Publication Date: 2020-11-24
OCEAN UNIV OF CHINA
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[0009] The first object of the present invention is to provide a method for constructing a marine microcosm system. In the method, by constructing a marine microcosm system composed of culture fluid, sediment, algae, protozoa, shrimp, and fish, it can Real and effective simulation of the microcosmic system in the surface sea area, so as to overcome the technical problems that the existing technology cannot restore the real environment and biological composition of the surface sea area, and further solve the problem that the existing technology cannot accurately evaluate the impact of oil and other pollutants on marine organisms in the real surface sea area. Technical Issues Affected by the System
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Embodiment 1
[0047] Prepare 18 5L beakers, wherein 200g of quartz sand, 0.5g of chitin, 0.5g of cellulose and 3Lf / 2 medium were added to each beaker.
[0048] Place the beaker in an environment at 20-22° C. with a light intensity of 3000 lux, and maintain a photoperiod of 12h (light): 12h (dark). Then, the beakers were inoculated with Platymonas helgolandicavar.tsingtaoensis, Phaeodactylum tricornutum Bohlin, Marine Chlorella, Karenia mikimotoi, and rib strips respectively. Skeletonemacostatum, Nitzschia closterium f.minutissima, Prorocentrum donghaiense, Alexandrium catenella, Chaetoceros curvisetus Cleve, Prorocentrum donghaiense Prorocentrum micans, sochrysis galbana and Melosira moniliformis and other 12 kinds of algae, and the initial cell concentration of algae in the beaker was 10 4 cells / mL. Then, after 4d, add Brachionus plicatilis (Brachionus plicatilis) and Chinese water flea (Calanus sinicus), and make the quantity of Brachionus plicatilis (Brachionus plicatilis) and Chinese ...
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The invention provides an oceanic microcosm system, an establishment method and a method for rapidly accessing the biotoxicity of oil spilling pollutants in ocean. In the establishment method of the oceanic microcosm system, a surface-layer sea area microcosm system can be really and effectively simulated by establishing the oceanic microcosm system consisting of a culture solution, sediments, algae, protists, prawns and fishes. In addition, the oceanic microcosm system has the advantages of being complete in food chain structure and capable of really simulating the surface-layer sea area microcosm system. The method for rapidly accessing the biotoxicity of the oil spilling pollutants in the ocean has the advantages of being quick and accurate, capable of really reflecting the influence of petroleum pollutants to the surface-layer sea area ecological environment and the like.
Description
technical field [0001] The invention relates to the field of assessment of the impact of oil pollutants on ecosystems, in particular to a marine microcosm system and a construction method, and a method for rapidly evaluating the biological toxicity of oil spill pollutants in the ocean. Background technique [0002] During the exploitation, refining, storage, transportation and use of petroleum and its products, due to artificial sewage or leakage, it is easy for petroleum to enter the marine environment, which will cause marine petroleum pollution. [0003] The oil film formed by petroleum on the sea surface weakens the energy of solar radiation penetrating into seawater, which will affect the photosynthesis of marine plants. At the same time, petroleum pollutants can interfere with the feeding, reproduction, growth, behavior and chemotaxis of organisms. Sea areas heavily polluted by oil can also lead to changes in the abundance and distribution of individual species, there...
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IPC IPC(8): A01K61/00A01K63/00A01G33/00
CPCA01G33/00A01K61/00A01K63/00Y02A40/80Y02A40/81
Inventor张焕新王影唐学玺赵新宇高莉媛曲同飞
OwnerOCEAN UNIV OF CHINA