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Application of perhexiline in the treatment of gastric cancer and intestinal cancer

A perhexiline and gastric cancer technology, applied in the field of medicine, can solve problems such as no perhexiline reports

Active Publication Date: 2019-12-20
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no reports on the application of perhexiline in other diseases such as gastric cancer and intestinal cancer

Method used

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  • Application of perhexiline in the treatment of gastric cancer and intestinal cancer
  • Application of perhexiline in the treatment of gastric cancer and intestinal cancer
  • Application of perhexiline in the treatment of gastric cancer and intestinal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Effect of Perhexiline on Gastric Cancer and Colorectal Cancer Cells and Their Tumor Formation

[0031] 1. Experimental materials

[0032] (1) Drug: perhexiline maleate is commonly used in clinical practice, and this example uses perhexiline maleate as the experimental drug.

[0033] The chemical structural formula of perhexiline maleate is as follows:

[0034]

[0035] (2) Cancer cells:

[0036] Gastric cancer cells: human gastric cancer cells HGC27 and MGC803;

[0037] Colorectal cancer cells: human colon cancer cells HCT 116 and human colon adenocarcinoma cells DLD-1.

[0038] (3) Commercially available apoptosis kits.

[0039] 2. Experimental grouping

[0040] (1) Control group: blank control, that is, the cancer cells were not treated with any drugs.

[0041] (2) Experimental group: cancer cells were treated with perhexiline maleate.

[0042] 3. The effect of perhexiline maleate on the apoptosis of gastric cancer and colorectal cancer cells detec...

Embodiment 2

[0060] Example 2 Effect of perhexiline on the proliferation of gastric cancer and colorectal cancer cells

[0061] 1. Experimental materials

[0062] (1) Drugs: Same as Example 1.

[0063] (2) Cancer cells: same as in Example 1.

[0064] (3) Commercially available MTS kits.

[0065] 2. Detect the activity of HGC27 and MGC803 cells, HCT 116 and DLD-1 cells after perhexiline maleate treatment by MTT method

[0066] (1) Pave gastric cancer and colorectal cancer cells in a 96-well plate. After they adhere to the wall, add medium containing 0 μM, 5 μM, 10 μM, and 20 μM perhexiline maleate to treat the cells, and then treat the cells at 24h and 48h , 72h by MTT method to detect HGC27 and MGC803 cells, HCT 116 and DLD-1 cell activity.

[0067] The specific method is as follows:

[0068]1) Digest HGC27 and MGC803 cells, HCT 116 and DLD-1 cells in the logarithmic growth phase with trypsin, and take 4×10 3 Cells were spread evenly into 96-well plates.

[0069] 2) After the cells ...

Embodiment 3

[0073] Example 3 Effect of Perhexiline on Metastasis of Gastric Cancer and Colorectal Cancer Cells

[0074] 1. Experimental materials

[0075] (1) Drugs: Same as Example 1.

[0076] (2) Gastric cancer cells: same as in Example 1.

[0077] (3) Transwell chambers with and without pre-coated Matrigel.

[0078] 2. The effect of perhexiline maleate treatment on the invasion and migration of HGC27 and MGC803 cells, HCT116 and DLD-1 cells was detected by Transwell chamber

[0079] (1) Spread gastric cancer and colorectal cancer cells in a six-well plate, and after they adhere to the wall, add 0 μM, 3 μM and 4 μM (or 5 μM) perhexiline maleate to treat gastric cancer cells for 48 hours, add 0 μM, 4 μM and Intestinal cancer cells were treated with 6 μM perhexiline maleate for 48 hours, and the effects of perhexiline maleate treatment on the invasion and migration of HGC27 and MGC803 cells, HCT 116 and DLD-1 cells were detected by Transwell chamber.

[0080] The specific method is as...

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PUM

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Abstract

The invention discloses an application of perhexiline in treating stomach cancers and intestinal cancers. After proofing by study, the perhxiline can obviously inhibit the growth, proliferation and coloning formation abilities of stomach cancer and colorectal cancer cells, induce the withering of the stomach cancer and colorectal cancer cells, inhibit the invasion and transfer abilities of the stomach cancer and colorectal cancer cells, and have obvious treatment effect on the stomach cancer and colorectal cancer, so that the perhxiline can be used as a medicine for treating the stomach cancer and intestinal cancer. The application has the advantages that a new application of the perhxiline is provided, the clinical application field of the perhxiline is widened, and a new chemical therapy medicine and a therapy path are provided for the therapy of stomach cancer and colorectal cancer.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it relates to the use of perhexiline in the treatment of gastric cancer and intestinal cancer. Background technique [0002] Gastric cancer and intestinal cancer are common gastrointestinal tumors in the world, ranking first among all kinds of malignant tumors in my country, and one of the main causes of death worldwide, and there are great difficulties in clinical treatment. [0003] Perhexiline is a calcium ion channel antagonist, which can inhibit Ca2+ influx, relax vascular smooth muscle, significantly dilate coronary arteries, increase coronary blood flow, and has a good effect on angina pectoris. It is mainly used clinically to treat angina pectoris. At the same time, it is also clinically used for ventricular arrhythmia, especially for patients who are ineffective to other antiarrhythmic drugs, this product can often work. Further studies have found that perhexiline c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4458A61P35/00
CPCA61K31/4458
Inventor 徐瑞华王运鞠怀强
Owner SUN YAT SEN UNIV CANCER CENT
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