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Actinobacillus pleuropneumoniae ghost vaccine

A technology for actinomycetes and pleuropneumonia, applied in the fields of microbiology and immunology, can solve the problems of increasing the cost of vaccine and epidemic prevention, economic losses in the pig industry, and wide distribution, so as to reduce the cost of epidemic prevention, good cross-immunity protection, and reduce The effect of production costs

Active Publication Date: 2017-05-31
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogen has many serotypes, can infect pigs of all ages, and has a wide distribution, which has brought great economic losses to the pig industry.
[0003] In the prior art, each serotype of APP inactivated vaccine can only protect against the same serotype of APP strains, which significantly increases the cost of vaccine prevention

Method used

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  • Actinobacillus pleuropneumoniae ghost vaccine
  • Actinobacillus pleuropneumoniae ghost vaccine
  • Actinobacillus pleuropneumoniae ghost vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of Actinobacillus pleuropneumoniae serotype 1 Shope strain bacterial shadow

[0022] 1. Culture of Bacteria

[0023] Since the Shope strain of APP serum type 1 has been cultured for 72 hours, the growth has entered the decline period, the reproduction is getting slower and slower, and the bacteria are prone to swelling or decay. shrink. Therefore, the present invention studies how to prepare bacteria ghosts with culture times of 12h, 24h, 48h, and 72h respectively.

[0024] APP serotype 1 Shope strain (Qiu Suoping, He Kongwang, Liu Zhongyong, Lin Zhixiong, Chen Wen. Establishment and application of PCR rapid typing system for Actinobacillus pleuropneumoniae. China Animal Quarantine, 2011, 28(8): 41- 45) Add THB medium containing 0.01% (mass percent concentration) NAD (nicotinamide adenine dinucleotide) from the cryopreservation tube, culture at 37°C for 14-18h, and then inoculate 2 % Inserted into THB medium containing 0.01% (mass percentage con...

Embodiment 2

[0041] Embodiment 2 Preparation effect of Actinobacillus pleuropneumoniae serotype 1 Shope strain bacterial shadow

[0042] According to the method in Example 1, the APP serum type 1 Shope strain cultivated for 12h, 24h, 48h, and 72h was adjusted to two concentrations of bacterial liquid (1 × 10 8 CFU / mL, 1×10 9 CFU / mL). Treat each condition (culture time, concentration) with the corresponding minimum inhibitory concentration NaOH solution at 37°C for 15, 30, 45, 60, and 75 minutes, centrifuge, take the precipitate and wash it repeatedly with sterile PBS buffer solution with pH 7.4 Resuspend after three trips. The volume ratio of bacterial solution to NaOH solution is 1:1. As a control bacterial solution, 100 μL of THB medium containing 0.01% NAD was added to 100 μL of each conditional bacterial solution. The lysis rate of bacteria under each treatment condition was detected, and scanning electron microscope (Scanning Electron Microscope, SEM) observation was carried out a...

Embodiment 3

[0050] Example 3 Immune efficacy test of Actinobacillus pleuropneumoniae ghost vaccine

[0051] 1. Vaccine Preparation

[0052] (1) Preparation of control vaccine

[0053] APP serotype 1 Shope strain, type 3 S1421 strain, type 5a K17 strain, and type 7 WF83 strain (Qiu Suoping, He Kongwang, Liu Zhongyong, Lin Zhixiong, Chen Wen. Establishment of PCR rapid typing system for Actinobacillus pleuropneumoniae and Application. China Animal Quarantine, 2011, 28 (8): 41-45) the bacterial liquid is inactivated by formaldehyde respectively, and then the inactivated bacterial liquid and mineral oil are mixed and emulsified according to the volume ratio of 1:3 to obtain serotype 1 , Type 3, Type 5a, Type 7 strains of oil emulsion inactivated vaccines (respectively abbreviated as S1 inactivated vaccines, S3 inactivated vaccines, S5a inactivated vaccines, S7 inactivated vaccines). The number of bacteria in each inactivated vaccine was 1×10 9 CFU / mL.

[0054] (2) Preparation of S1 ghost ...

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Abstract

The invention provides an actinobacillus pleuropneumoniae ghost vaccine, belonging to the field of microbiology and immunology. The actinobacillus pleuropneumoniae ghost vaccine contains actinobacillus pleuropneumoniae serotype 1 Shope ghost. The actinobacillus pleuropneumoniae ghost vaccine can completely prevent attack of the same serotype actinobacillus pleuropneumoniae, provide good cross immunity protection for other serotypes, and obviously reduce the cost of vaccines and epidemic prevention.

Description

technical field [0001] The invention belongs to the field of microbiology and immunology, and in particular relates to a ghost vaccine of Actinobacillus pleuropneumoniae. Background technique [0002] Porcine contagious pleuropneumonia (PCP) is a highly contagious and fatal respiratory disease caused by Actinobacillus pleuropneumoniae (APP). The pathogen has many serotypes, can infect pigs of all ages, and has a wide distribution range, which has brought great economic losses to the pig industry. [0003] In the prior art, each serotype of APP inactivated vaccine can only protect against the attack of the same serotype of APP strains, which significantly increases the cost of vaccine prevention. Contents of the invention [0004] The object of the present invention is to provide a kind of Actinobacillus pleuropneumoniae ghost vaccine, which can not only fully protect the attack of the same serotype Actinobacillus pleuropneumoniae, but also provide good cross-immune protec...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61P31/04A61P37/04C12N1/06C12R1/01
CPCA61K39/0208A61K2039/521A61K2039/552C12N1/06
Inventor 倪艳秀何孔旺王丹丹祝昊丹周俊明吕立新俞正玉茅爱华
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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