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foxc1 gene h446hg mutant and its application

A mutant and gene technology is applied in the application field of children's acute lymphoblastic leukemia screening kit, and achieves the effect of great practical value and application prospect

Active Publication Date: 2019-11-26
NANJING CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved: the purpose of the present invention is to solve the problem of whether there is a relevant mutation in T cells and the occurrence of ALL in children, to provide a FOXC1 gene H446HG mutant and its application, and to compare and analyze children with ALL through whole exome sequencing technology T cell mutations and normal control T cell mutations, screen out ALL-specific T cell mutations, and provide a new method for the early diagnosis of children with ALL in clinical practice

Method used

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  • foxc1 gene h446hg mutant and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Selection of research subjects and collection of bone marrow samples

[0028] The bone marrow samples of all cases were from new-onset leukemia patients clearly diagnosed by MICM typing in the Department of Hematology and Oncology, Children's Hospital Affiliated to Nanjing Medical University, and 1-2 mL of fresh bone marrow was collected by sternal puncture.

[0029] All control bone marrow samples were obtained from patients with new-onset idiopathic thrombocytopenic purpura (ATP).

[0030] Sample collection and DNA extraction:

[0031] 1). Collect 1-2 mL of bone marrow from children with new-onset ALL and bone marrow (control) from new-onset ITP patients, and use the Ficoll method to separate infiltrating lymphocytes in a biological safety cabinet.

[0032] 2). Take two 15mL centrifuge tubes, first add 5mL of Ficoll-paque PLUS reagent to each tube, and then use a dropper to slowly superimpose 5mL of blood on the layered liquid surface along the tube wall, ...

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Abstract

The invention discloses an FOXC1 gene H446HG mutant and application thereof. The mutant is characterized in that one glycine is inserted behind amino acid histidine at position 446, and the mutant can be used for preparing a kit for screening childhood acute lymphocytic leukemia. According to the FOXC1 gene H446HG mutant and the application, T cell mutations relative to occurrence of the childhood acute lymphocytic leukemia is screened from a genetics angle firstly, a new direction is supplied for early screening the childhood acute lymphocytic leukemia from a molecular level, and the application of the FOXC1 gene H446HG mutations in preparing the kit for screening the childhood acute lymphocytic leukemia is supplied, so that the FOXC1 gene H446HG mutant has great practical value and application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of molecular biology gene detection, and in particular relates to the FOXC1 gene H446HG mutant and its application in children's acute lymphoblastic leukemia screening kit. Background technique: [0002] Acute leukemia is one of the major diseases that endanger children's health. It is a malignant proliferative disease in which hematopoietic stem cells are blocked at a certain stage during the differentiation process. Among them, acute lymphoblastic leukemia (ALL) is the most common in children. malignant blood diseases, accounting for about 75% of leukemia. With the continuous improvement of the diagnosis and treatment of children with ALL, the application of multi-drug combination therapy, supportive therapy and hematopoietic stem cell transplantation, its 5-year disease-free survival rate has exceeded 80-90%, and it has become a curable malignant tumor. However, there are still quite a few ALL patients ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12C12Q1/6886C12N15/11
CPCC07K14/4702C12Q1/6886C12Q2600/156C12Q2600/166
Inventor 方拥军薛瑶王娅萍康美云戎留成王美林
Owner NANJING CHILDRENS HOSPITAL