A non-differentiation expansion anti-aging medium for human umbilical cord/adipose mesenchymal stem cells

A technology of stem cells and human umbilical cord, applied in the field of stem cells, can solve the problems of apoptosis and senescence of adult stem cells, and achieve the effect of resisting cell senescence and apoptosis, preventing senescence and pathological changes, and preventing non-directional differentiation.

Inactive Publication Date: 2020-03-24
北京殷氏细胞生物科技集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention proposes a non-differentiation and expansion anti-aging culture medium for human umbilical cord / fat mesenchymal stem cells, which solves the problem of easy differentiation of serum-free culture medium used for culturing mesenchymal stem cells in the prior art, and easy aging of adult stem cells. The problem of apoptosis

Method used

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  • A non-differentiation expansion anti-aging medium for human umbilical cord/adipose mesenchymal stem cells
  • A non-differentiation expansion anti-aging medium for human umbilical cord/adipose mesenchymal stem cells
  • A non-differentiation expansion anti-aging medium for human umbilical cord/adipose mesenchymal stem cells

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Embodiment 1

[0053] A non-differentiation expansion anti-aging medium for human umbilical cord / adipose mesenchymal stem cells, comprising cytokines, vitamins, and chemical small molecules, wherein the contents of each component are as follows:

[0054]

[0055]

[0056]

[0057] The culture medium of the present invention also includes DMEM / F12 basal culture medium added with ITS supplement. ITS is insulin-transferrin-sodium selenite medium additive, the configuration method of basal medium: after mixing DMEM and F12 according to the ratio of solution volume ratio 1:1, add 1% ITS to form again, DMEM / The F12 medium is provided by Gibco Company, the product number is 11320-033, and the ITS is the product of Sigma Company, the product number is I1884.

Embodiment 2

[0059] A non-differentiation expansion anti-aging medium for human umbilical cord / adipose mesenchymal stem cells, comprising cytokines, vitamins, and chemical small molecules, wherein the contents of each component are as follows:

[0060]

[0061]

[0062]

[0063] The culture medium of the present invention also includes DMEM / F12 basal culture medium added with ITS supplement. ITS is insulin-transferrin-sodium selenite medium additive, the configuration method of basal medium: after mixing DMEM and F12 according to the ratio of solution volume ratio 1:1, add 1% ITS to form again, DMEM / The F12 medium is provided by Gibco Company, the product number is 11320-033, and the ITS is the product of Sigma Company, the product number is I1884.

Embodiment 3

[0065] A non-differentiation expansion anti-aging medium for human umbilical cord / adipose mesenchymal stem cells, comprising cytokines, vitamins, and chemical small molecules, wherein the contents of each component are as follows:

[0066]

[0067]

[0068] The culture medium of the present invention also includes DMEM / F12 basal culture medium added with ITS supplement. ITS is insulin-transferrin-sodium selenite medium additive, the configuration method of basal medium: after mixing DMEM and F12 according to the ratio of solution volume ratio 1:1, add 1% ITS to form again, DMEM / The F12 medium is provided by Gibco Company, the product number is 11320-033, and the ITS is the product of Sigma Company, the product number is I1884.

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Abstract

The invention provides an undifferentiated anti-aging amplification culture medium for human umbilical cord / adipose tissue-derived stem cells. The problem in the prior art that a serum-free medium used for culturing mesenchymal stem cells is easy to differentiate and easily causes aging and apoptosis of adult stem cells is solved. The undifferentiated anti-aging amplification culture medium for human umbilical cord / adipose tissue-derived stem cells comprises cell factors, vitamins and chemical small molecules, and the culture system comprises multiple chemical small molecules and biomacromolecules and has the effects of improving physiological metabolism and proliferation and growth potential of cells, resisting oxidative stress response, scavenging free radicals, preventing DNA mutation, protecting mitochondria, reducing molecular wastes in the cells and activating longevity genes and telomerase.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a non-differentiation expansion anti-aging culture medium for human umbilical cord / fat mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells including umbilical cord / placenta (UC-MSC), bone marrow (BM-MSC) and adipose-derived mesenchymal stem cells (AD-MSC) have the ability of self-renewal, can expand in large quantities, and will also age and undergo apoptosis. This is different from embryonic stem cells (ESC), induced pluripotent stem cells (iPS). Second, AD-MSC has multi-directional differentiation potential and can form a variety of terminal tissue cells, such as nerve cells, retinal cells, liver cells, gastrointestinal tissue, adipose tissue, muscle blood vessels, blood, skin hair, bone and chondrocytes Wait. These characteristics indicate that mesenchymal stem cells can differentiate into different types of cells under certain culture conditions....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N5/0668C12N2500/30C12N2500/34C12N2500/35C12N2500/38C12N2500/46C12N2501/105C12N2501/11C12N2501/115C12N2501/12C12N2501/125C12N2501/135C12N2501/15C12N2501/16C12N2501/165C12N2501/235C12N2501/727
Inventor 黄兵殷勤伟
Owner 北京殷氏细胞生物科技集团有限公司
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