Carbofuran assay determination kit and application thereof

A kit, the technology of Carboweil, is applied in the field of food safety detection immunoassay to achieve the effects of improved sensitivity, easy operation and production, and good reproducibility

Inactive Publication Date: 2017-05-31
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the current prior art, there is still a lack of a highly sensitive method for detecting carbofuran using biological barcode technology

Method used

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  • Carbofuran assay determination kit and application thereof
  • Carbofuran assay determination kit and application thereof
  • Carbofuran assay determination kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation method of carbofuran analysis and determination kit

[0048] S11, Preparation of carbofuran enzyme-labeled colloidal gold composite probe

[0049] Gold Nanoparticle Synthesis Method

[0050] Add 98mL of deionized water to the siliconized beaker respectively, and then add 2mL of 50mM chloroauric acid stock solution respectively, so that the concentration of chloroauric acid is 1mM, stir on a magnetic stirrer at 1000rpm / min, heat at 250°C, continue to boil after the liquid boils Boil for 2 minutes; absorb 10mL of 38.8mM trisodium citrate, quickly add it to the beaker at one time, keep the stirring speed and heating temperature constant, and continue to boil for 6 minutes. It can be found that the color of the solution gradually changes from colorless to purple, and then into wine red. According to the different reducing doses of trisodium citrate added, it finally turns into different colors. After the liquid is cooled, deionized water is used to restore the ...

Embodiment 2

[0074] Kit use method of the present invention

[0075] The specific operation of the Carbofuran enzyme-labeled colloidal gold composite probe amplification immunoassay kit prepared in the above implementation case 1 is as follows:

[0076] S21. Take out the kit from the refrigerator at 4°C, and equilibrate at room temperature for 10 minutes;

[0077] S22. Carbofuran standard substances (10.0ng L -1 -40 μg L -1 ) and 20 μL of each sample obtained after pretreatment, set up a repetition, then add 20 μL of magnetic nanoprobes to each well, fully oscillate and mix with a micro shaker, and then place the temperature at 37 ° C and a relative humidity of 70% constant temperature Incubate for 1 h in a constant humidity incubator;

[0078] S23, washing with PBST solution three times, dehybridization to obtain a competition complex;

[0079] S24. Add 50 μL of the reaction substrate TMB to the competition reaction complex, react at 37°C for 15 minutes, then add 50 μL of stop solution ...

experiment example 1

[0082] The methodological examination result of kit of the present invention

[0083] The kit prepared in Example 1 was identified according to the common verification procedures in this field, and the results are shown in Table 1.

[0084] The methodological testing result of test kit of the present invention of table 1

[0085]

[0086] The above results show that the accuracy, specificity, precision, sensitivity and stability of the "Carbofuran Colloidal Gold Composite Probe Combined with HRP Immunoassay Assay Kit" are fully qualified.

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Abstract

The invention relates to the technical field of food safety inspection immunoassay, and in particular relates to a carbofuran assay determination kit. The kit comprises a carbofuran standard substance, a carbofuran nanoprobe, a carbofuran enzyme-labeled colloidal gold complex probe, a catalytic substrate and a stop buffer. According to the kit disclosed by the invention, the substrate TMB is catalyzed to develop color by enzymes, the sensitivity is greatly improved, and a sensitive, rapid and reliable basis is provided for detection of the carbofuran in vegetables and fruits.

Description

technical field [0001] The invention relates to the technical field of food safety detection and immunoassay, in particular to a carbofuran analysis and determination kit and its application. Background technique [0002] Pesticides and veterinary drugs are indispensable and important means of production for agricultural production, and play an important role in ensuring agricultural and food production and safety. In the foreseeable future, chemical pesticides and veterinary drugs will still be an indispensable means of ensuring the quality and safety of agricultural products. Residues of pesticides and veterinary drugs in food and their safety assessment are widely concerned by scientific researchers and the public. However, if it is used unreasonably for a long time, it will cause environmental pollution, ecological damage and harm to human health. Therefore, the establishment of efficient, fast and low-cost pesticide residues in soil and water has become a top priority...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/543
CPCG01N33/577G01N33/54313G01N33/54326G01N33/54346G01N33/94G01N2430/10
Inventor 金茂俊王静杜鹏飞金芬佘永新邵华郑鹭飞王珊珊
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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