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A kind of preparation method of chitosan-agar oligosaccharide porous ball beads hemostatic material

A technology of hemostatic material and oligosaccharides, which is applied in pharmaceutical formulation, surgery, drug delivery, etc., to achieve the effect of excellent swelling effect, large porosity, and simple preparation process

Active Publication Date: 2019-11-12
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

WoundStat hemostatic agent was approved by the US FDA in 2008 and was widely used in the military in the same year, but it was later found that there was a risk of peripheral thrombus entering the blood circulation system. Based on biosafety considerations, the US military announced in 2009 that it would ban the permanent use of WoundStat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of chitosan-agar oligosaccharide porous ball beads hemostatic material comprises the following steps:

[0021] S1. Dissolve chitosan (molecular weight 200 kDa, degree of deacetylation 95%) with 1% (V / V) acetic acid aqueous solution to prepare 2% (W / V) chitosan solution; dissolve agar oligosaccharides with purified water (molecular weight 2000 Da), prepare 0.5% (W / V) agar oligosaccharide aqueous solution; take an equal volume of the above two solutions and mix them to obtain a chitosan-agar oligosaccharide composite solution;

[0022] S2. Add 1,3-diglycidyl ether glycerin (2%, V / V) to isopropanol / isooctanol (volume ratio 4:6) compound alcohol, place it in an ultrasonic instrument, and ultrasonic power 130W; add the above-mentioned chitosan-agar oligosaccharide compound solution dropwise into the compound alcohol with a No. 8 needle (the volume ratio of the compound solution to the compound alcohol solution is 1:5), stop the ultrasound after the add...

Embodiment 2

[0026] The preparation method of chitosan-agar oligosaccharide porous ball beads hemostatic material comprises the following steps:

[0027] S1. Dissolve chitosan (molecular weight 300 kDa, degree of deacetylation 85%) with 2% (V / V) acetic acid aqueous solution to prepare 2.5% (W / V) chitosan solution; dissolve agar oligosaccharides with purified water (Molecular weight 3000 Da), prepare 1.0% (W / V) agar oligosaccharide aqueous solution; take an equal volume of the above two solutions and mix them to obtain a chitosan-agar oligosaccharide composite solution;

[0028] S2. Add 1,3-diglycidyl ether glycerin (1.5%, V / V) to isopropanol / isooctanol (volume ratio 4:6) complex alcohol, place it in an ultrasonic instrument for ultrasonication, and the ultrasonic power 135W; add the above-mentioned chitosan-agar oligosaccharide compound solution dropwise into the compound alcohol with a No. 8 needle (the volume ratio of the compound solution to the compound alcohol solution is 1:4), stop t...

Embodiment 3

[0032] The preparation method of chitosan-agar oligosaccharide porous ball beads hemostatic material comprises the following steps:

[0033] S1. Dissolve chitosan (molecular weight 400 kDa, degree of deacetylation 90%) with 2% (V / V) acetic acid aqueous solution to prepare 3% (W / V) chitosan solution; dissolve agar oligosaccharides with purified water (Molecular weight 2000 Da), prepare 1.5% (W / V) agar oligosaccharide aqueous solution; take equal volumes of the above two solutions and mix well to prepare a chitosan-agar oligosaccharide composite solution;

[0034] S2. Add 1,3-diglycidyl ether glycerin (1%, V / V) to isopropanol / isooctanol (volume ratio 4:6) compound alcohol, place it in an ultrasonic instrument for ultrasonication, and the ultrasonic power 140W; add the above-mentioned chitosan-agar oligosaccharide complex solution dropwise into the complex alcohol with a No. 8 needle (the volume ratio of the complex solution to the complex alcohol solution is 1:5), stop the ultra...

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Abstract

The invention discloses a preparation method of a chitosan-agar oligosaccharide porous ball hemostatic material. The method comprises the following steps of: S1, preparing a chitosan-agar oligosaccharide compound solution; S2, adding a crosslinking agent 1,3-diepoxy glyceryl ether glycerinum into a compound alcohol solution, dropwise adding the chitosan-agar oligosaccharide compound solution into the compound alcohol solution containing the 1,3-diepoxy glyceryl ether glycerinum under the ultrasonic power of 130-150W, after dropwise addition, stopping ultrasonic treatment, and stirring the mixture for 30min at 600-800rpm; and S3, filtering balls, and washing, freezing and drying the balls. The hemostatic material prepared by the invention is good in biocompatibility and safe and non-toxic, can quickly accelerate blood clotting and has efficient hemostatic activity, has a certain volume, and is unlikely to enter blood to induce a thrombus risk. Meanwhile, the material has excellent wound adaptability, and is in particular suitable for bleed stopping of wounds which are deep, narrow and irregular. The preparation disclosed by the invention is simple in preparation process and easy for industrial production.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a preparation method of a chitosan-agar oligosaccharide porous ball bead hemostatic material. Background technique [0002] In times of war, more than 80% of casualties are loss of life due to traumatic blood loss; in peaceful times, massive blood loss due to trauma caused by natural disasters, accidents and other emergencies is also the main cause of disability and death. Traditional hemostatic cotton gauze, bandages, etc. have unsatisfactory hemostatic effects on irregularly shaped, deep, narrow, ruptured arteries and other wounds; although hemostatic powder has excellent wound applicability, it is easy to enter the blood and block the flow of peripheral arteries to form thrombus. Therefore, a fast, safe and efficient hemostatic material suitable for on-site and clinical first aid is needed to replace traditional hemostatic materials. [0003] Chitosan is a natural biologic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/001A61L24/0036A61L24/08A61L2400/04C08L5/08C08L5/12
Inventor 胡章李思东洪鹏志李程鹏孔松芝黄娜程瑜
Owner GUANGDONG OCEAN UNIVERSITY
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