Method for simultaneous detection of fat-soluble vitamins and water-soluble vitamins in feed
A technology for fat-soluble vitamins and water-soluble vitamins, applied in the field of high-performance liquid chromatography detection, can solve the problems of incomplete detection at the same time, multiple sources of vitamins, complex components, etc., to achieve optimized detection efficiency and accuracy, accurate and reliable results, and high efficiency separation effect
Active Publication Date: 2020-04-28
NEW HOPE LIUHE +1
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[0006] The purpose of the present invention is to overcome the disadvantages in the prior art that complex ingredients in compound feed, many sources of vitamins, and low content cannot be completely detected in one detection, and provide a method for simultaneously detecting fat-soluble vitamins and vitamins in feed. water soluble vitamins
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[0034] The present invention will be further described in detail below in conjunction with test examples and specific embodiments. However, it should not be understood that the scope of the above subject matter of the present invention is limited to the following embodiments, and all technologies realized based on the content of the present invention belong to the scope of the present invention.
[0035] 1 Preparation of instruments, reagents and sample materials
[0036] (1) Agilent 1260-6460 liquid chromatography-triple quadrupole tandem mass spectrometer equipped with electrospray ion source ESI (Agilent Corporation, USA).
[0037] (2) AB-135 electronic analytical balance (Mettler Toledo Shanghai Co., Ltd.).
[0038] (3) LD5-2B centrifuge (Beijing Jingli Centrifuge Co., Ltd.).
[0039] (4) KQ-500DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).
[0040] (5) Vortex mixer MS3 (IKA, Germany).
[0041] (6) Acetonitrile, methanol (chromatographically pure,...
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The invention discloses a method for simultaneously detecting fat-soluble vitamins and water-soluble vitamins in feed. The method comprises (1) preparing a multivitamin standard solution comprising at least two or more of the following vitamins such as VB1, VB3, VB6, VB2, VB7, VB12, vitamin A acetate, vitamin D3 and vitamin E acetate and acquiring a standard curve equation through a HPLC-MS / MS detection standard solution, (2) weighing 5-10g of a sample, adding the sample into a volumetric flask and preferably into 100 ml of a brown volumetric flask, adding an extraction solution into the brown volumetric flask, carrying out ultrasonic extraction for 10-30min, carrying out cooling, volume metering and centrifugation, carrying out filtration through an organic phase filter machine having thickness of 0.22 microns, adding the filtrate into a detection device and carrying out an analysis test through the gradient elution program and mass spectrometry detection parameters used in the step 1, and (3) calculating multivitamin contents in the sample according to the test results in the steps 1 and 2. The method realizes one-step continuous detection, analysis and treatment on a plurality of vitamins in complex compound feed.
Description
technical field [0001] The invention relates to a high-performance liquid chromatography (HPLC) detection method, in particular to a method for simultaneously detecting multiple vitamins in feed by high-performance liquid chromatography combined with mass spectrometry-mass spectrometry, including the simultaneous detection of fat-soluble vitamins and water-soluble vitamins. Background technique [0002] Due to the great difference in the solubility of water-soluble vitamins and fat-soluble vitamins, there are few reports on the methods for their simultaneous determination. [0003] Semahat Kucukkolbasi et al. have reported a method for simultaneously determining the contents of water-soluble vitamins and fat-soluble vitamins in multivitamin tablets. The method adopts trifluoroacetic acid-methanol (TFA-MeOH) system to extract vitamins in multivitamin tablets, TFA-MeOH system is mobile phase gradient elution, separates vitamins in Phenomenex Gemini C18 column, adopts diode arr...
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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 朱正鹏梁玉树何开蓉易锡斌张遨然汪春霞王虎周丽黄李蓉梁秋菊
Owner NEW HOPE LIUHE