Establishment method of diet-induced SD rat diabetic animal model

Abstract

The invention is mainly applied to medical research in which an SD rat is used as a research subject. A human diabetic pathogenetic feature is simulated through high-fat and high-sugar feed, and a method for constructing a pure high-fat and high-sugar diet induced diabetic animal model is provided. A main research method comprises the following steps that 40 SPF-level healthy SD rats are divided into a common feed control group (C) and a high-fat and high-sugar feed modeling group (CH), each group consists of 20 rats, and half are female and half are male. Energy supply ratios of common feed are as follows: 63.02 percent of carbohydrate, 24.93 percent of protein and 12.05 percent of fat; energy supply ratios of the high-fat and high-sugar feed are as follows: 66.14 percent of carbohydrate, 10.33 percent of protein and 23.53 percent of fat. According to the method, the construction of the pure high-fat and high-sugar diet diabetic animal model by inducing through feeding the rats with the high-fat and high-sugar feed for a long time is adopted; the method can be used for better simulating the pathogenetic and development features of diabetes mellitus type 2 of a crowd, and compared with chemical diabetic modeling, the method has more scientific significance.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a method for establishing an animal model of SD rat diabetes induced by diet. Background technique [0002] Because there are species differences among different animals, the cost of rodents is relatively low, and the operation is convenient and easy. Therefore, rodents are often selected as modeling objects in animal models of diabetes. 90% of patients in our country belong to type Ⅱ diabetes. For the structure of type Ⅱ diabetes animal model, most of them choose rodents. Primates, minipigs and other species of mammals are closer to humans, but due to the high cost of research and difficult operation, there are few studies. The currently widely used diabetic animal models are mainly divided into: experimental diabetic animal models, spontaneous diabetic animal models, transgenic and gene-proposed diabetic animal models. The most common experimental diabetic animal model is the diabetic...

AI Technical Summary

Problems solved by technology

[0006] The present invention uses SD rats as experimental animals to simulate the bad eating habits of long-term high-fat and high-sugar population of type 2 diabetes, and provides a method for inducing a simple high-fat and high-sugar diet to feed an animal model of type II diabetes. It aims to solve the problems of large side effects and poor stability caused by various chemical modeling to animals