This invention belongs to the field of
traditional Chinese medicine, specifically relating to a purified
flavonoid from
Sophora flavescens, its preparation method, and its application. Using
Sophora flavescens as
raw material, this invention extracts and separates purified
flavonoid KSF50, and uses liquid
chromatography-
mass spectrometry (LC-MS / MS) to analyze and identify its components, determining its main functional groups and composition. The purified
flavonoid from
Sophora flavescens mainly contains the following six flavonoid components:
daidzein, gentiopicrin,
matrine O, trifolin stigmosiderin,
matrine, and sophoridine. Furthermore, based on a high-
sugar diet-induced *C. elegans* model and a high-
sugar-induced 3T3-L1 preadipocyte model, this invention provides evidence that
Sophora flavescens flavonoids can
delay the aging of *C. elegans* by regulating
lipid metabolism disorders, providing a theoretical basis for its application in
medicine,
cosmetics, and health functional foods.