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Low-temperature plasma treatment method for improving β-mannanase activity

A low-temperature plasma and mannanase technology, applied in the field of plasma treatment, can solve the problems of insufficient activity and stability, low substrate degradation rate, etc., and achieve the improvement of β-mannanase activity and stability, Improve activity and stability, and shorten the treatment cycle

Active Publication Date: 2019-10-01
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a biological enzyme preparation, β-mannanase also has problems such as its catalytic activity is greatly affected by external pH value, temperature and other process conditions, its activity and stability are not high enough, and its substrate degradation rate is low.

Method used

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  • Low-temperature plasma treatment method for improving β-mannanase activity
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  • Low-temperature plasma treatment method for improving β-mannanase activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Weigh 0.200 g of β-mannanase, set a horizontal support platform transparent up and down in the low-temperature plasma chamber, set an expanded thin planar porous network on the support platform, and then place the β-mannan The enzyme is distributed thinly and evenly, and then the chamber door of the low-temperature plasma treatment device is closed.

[0042]Use the vacuum pump configured in the system to evacuate the above chamber, and use argon to wash or replace the reaction chamber for 2-3 times. In this implementation, argon is selected as the working gas, and the working conditions are: gas pressure 20Pa, discharge power 100W, and processing time 4min. After the scrubbing process is over, open the intake valve, and when the pressure and airflow of the atmosphere to be treated are stabilized, turn on the discharge switch for glow discharge, and start the countdown when the discharge power is turned on. After the treatment is completed, close the discharge switch, v...

Embodiment 2

[0055] Weigh 0.200 g of β-mannanase, set a horizontal support platform transparent up and down in the low-temperature plasma chamber, set an expanded thin planar porous network on the support platform, and then place the β-mannan The enzyme is distributed thinly and evenly, and then the chamber door of the low-temperature plasma treatment device is closed.

[0056] Use the vacuum pump configured in the system to evacuate the above chamber, and use nitrogen to wash or replace the reaction chamber for 2-3 times. In this implementation, nitrogen is selected as the working gas, and the working conditions are: gas pressure 65Pa, discharge power 100W, and processing time 4min. After the scrubbing process is over, open the intake valve, and when the pressure and airflow of the atmosphere to be treated are stabilized, turn on the discharge switch for glow discharge, and start the countdown when the discharge power is turned on. After the treatment is completed, close the discharge sw...

Embodiment 3

[0059] Weigh 0.200 g of β-mannanase, set a horizontal support platform transparent up and down in the low-temperature plasma chamber, set an expanded thin planar porous network on the support platform, and then place the β-mannan The enzyme is distributed thinly and evenly, and then the chamber door of the low-temperature plasma treatment device is closed.

[0060] Use the vacuum pump configured in the system to evacuate the above chamber, and use oxygen to wash or replace the reaction chamber for 2-3 times. In this implementation, oxygen is selected as the working gas, and the working conditions are: gas pressure 95Pa, discharge power 100W, and processing time 4min. After the scrubbing process is over, open the intake valve, and when the pressure and airflow of the atmosphere to be treated are stabilized, turn on the discharge switch for glow discharge, and start the countdown when the discharge power is turned on. After the treatment is completed, close the discharge switch...

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Abstract

The invention relates to a low-temperature plasma treatment method for improving the activity of beta-mannase. The method comprises the following steps of: arranging a bracket table in a treatment chamber of a low-temperature plasma treatment device, arranging a porous material on the bracket table, and putting beta-mannase on the porous material; and emptying the treatment chamber, introducing work gas, and performing discharge treatment for 2-12 min, wherein the gas pressure is 20-95Pa, and the discharge power is 50-300W. The method provided by the invention can effectively improve the activity of beta-mannase and improve the treatment effect, and is high in treatment efficiency. The low-temperature plasma treatment process is a dry state treatment method without using chemicals, so that the method is green and environmental-friendly as well as energy-saving and emission-reducing, and has very wide market prospects.

Description

technical field [0001] The invention relates to the technical field of biological enzyme modification processing, in particular to a low-temperature plasma treatment method for improving the activity of β-mannanase. Background technique [0002] β-mannanase is an endolytic enzyme that hydrolyzes mannan oligosaccharides and mannan polysaccharides (mannan, glucomannan, galactomannan) with β-1,4-D-mannopyranose as the main chain hydrolase. β-mannanase comes from a wide range of sources, including bacteria, fungi, actinomycetes, plants and molluscs. Among them, microorganisms are the main source of producing β-mannanase. Bacillus, Pseudomonas, Vibrio in bacteria, Aspergillus, Trichoderma, Yeast, Penicillium, Polypore, Sclerotinia and Streptomyces in Actinomycetes all produce β-mannanase common taxa. [0003] As a biocatalyst, β-mannanase has significant advantages such as high efficiency and specificity, mild reaction conditions, and no pollution to the environment, and has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N13/00C12N9/24
Inventor 龙家杰祁丽
Owner SUZHOU UNIV