A method for simultaneous detection of multiple adulterants in milk powder

Abstract

The invention belongs to the technical field of food analysis, and more specifically provides a simultaneous detection method of plurality of dopants in milk powder. According to the simultaneous detection method of plurality of dopants in milk powder, a raman spectrometer is used, and the dopants comprise melamine, dicyandiamide, and sodium sulfocyanate. The simultaneous detection method comprises steps of preparation of chitosan-SERS paper chromatography, pretreatment of milk powder samples, separation of paper chromatography, and SERS detection. A preparation method of self-made SERS paper chromatography is simple; cost is low; separation effect is excellent; the raman spectrometer is convenient to carry; the simultaneous detection method is suitable for on-site rapid detection; the pretreatment process of the milk powder samples is simple, and only filtering is needed; an obtained non-blank spectrogram is compared with the standard raman spectrograms of the above three dopants point by point so as to determine whether a detected sample contains the dopants; no reference experiment is needed; the toxicity of a selected developing solvent is relatively low; the developing solvent is friendly to the environment; separation of the dopants is impossible to realize with other developing solvents; separation detection time of the samples is shorter than 25min; and on-site rapid detection can be realized.

Description

technical field [0001] The invention relates to the technical field of food analysis, in particular to a method for simultaneously detecting multiple adulterants in milk powder. Background technique [0002] The method commonly used in the milk powder industry to detect protein content is the Kjeldahl method. The Kjeldahl method does not directly detect the protein content, but calculates the egg self-quality content by measuring the nitrogen content. Melamine is an important raw material for the organicization of nitrogen heterocycles and an important post-processing product of urea. The nitrogen content of melamine is as high as 66%. If it is mixed into milk products, it can cause the false impression that the egg self-quality content reaches the standard in the test. At the beginning of 2007, many cats and dogs were poisoned and died in the United States because of the illegal addition of melamine. The U.S. Food and Drug Administration (FDA) found that eating melamine ...

AI Technical Summary

Problems solved by technology

The nitrogen content of melamine is as high as 66%. If it is mixed into milk products, it can cause the false impression that the egg quality content is up to standard in the test.

Chinese patent document CN103878382A discloses a self-made special gold nanocrystal to successfully detect melamine in dairy products, but the preparation of this kind of substrate is difficult, the cost is high, and the liquid SERS substrate is unstable, so it is only suitable for preparation and application in the laboratory

In addition, there are not many reports on the detection of new additives such as dicyandiamide and sodium thiocyanate, and there are few methods for simultaneous detection of multiple additives

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