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Tissue culture production method of Anoectochilus roxburghii adventitious shoots

A production method and technology of clematis, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low labor efficiency of aseptic operation, low survival rate of single seedling transplanting, large workload, etc. , to achieve the effect of improving aseptic operation efficiency, overall vitality and resistance, and labor efficiency

Inactive Publication Date: 2017-07-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When inducing rooting, this method needs to cut clustered buds into individual buds multiple times in the aseptic workbench, which has a large workload, low efficiency of aseptic operation, and low labor efficiency for subsequent planting of single seedlings
In addition, the transplanting survival rate of single seedlings is also low

Method used

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  • Tissue culture production method of Anoectochilus roxburghii adventitious shoots
  • Tissue culture production method of Anoectochilus roxburghii adventitious shoots
  • Tissue culture production method of Anoectochilus roxburghii adventitious shoots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The material is made of Taiwan clematis seedlings (obtained by conventional methods, or purchased from many manufacturers). After confirming that there is no fungus contamination, perform appropriate cutting operations in a sterile workbench to obtain stem segments with single nodes (lateral buds, axillary buds) and stem end materials with terminal buds.

[0028] The stem segments with a single node and the stem end materials with terminal buds were inoculated and cultured on the "cluster bud induction medium" containing different concentrations of BA to induce the formation of cluster buds. The results are shown in Table 1.

[0029] Table 1 The effect of BA on the induction of cluster buds of A. formosana

[0030]

[0031] Note: Cultivate 5 bottles of each concentration of BA, with 5 stem segments or stem ends in each bottle, and the culture time is 60 days; the concentration of NAA in the medium is 1.0 mg / L; the data in the table are the corresponding number of ste...

Embodiment 2

[0041] The bottle seedlings of A. yunnanensis are used as materials (this material is cultivated from wild species collected locally in Mengla, Yunnan by conventional tissue culture methods). Carry out suitable cutting operations in a sterile bench to obtain stem segments with individual nodes (lateral buds, axillary buds) and stem end material with terminal buds. The stem segments with a single node and the stem end materials with terminal buds were inoculated and cultured on the "cluster bud induction medium" containing different concentrations of BA to induce the formation of cluster buds. The results are shown in Table 3.

[0042] Table 3 Effects of BA on induction of cluster buds of A. yunnanensis

[0043]

[0044] Note: Cultivate 5 bottles of each concentration of BA, with 5 stem segments or stem ends in each bottle, and the culture time is 60 days; the concentration of NAA in the medium is 1.0 mg / L; the data in the table are the corresponding number of stem segments or...

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Abstract

The invention relates to a tissue culture production method of Anoectochilus roxburghii adventitious shoots. The method comprises the following steps: S1: aseptic Anoectochilus roxburghii bottle seedling branches are cut into stem segments of single node and stem terminals with a terminal bud, and the stem segments and stem terminals are inoculated to an adventitious bud induction medium for forming adventitious buds; S2: adventitious buds obtained in the step S1 are cut into separate buds, and the buds are inoculated to a single bud growth medium for forming branches with 3-5 nodes; S3: branches obtained in the step S2 are cut into stem segments of single lateral bud and stem terminals with terminal buds, and the stem segments and the stem terminals are inoculated to an adventitious bud induction medium for inducing and forming adventitious buds; S4: the step S2 and the step S3 are circulated in order for several times till adventitious buds of predetermined quantity are obtained, adventitious buds are inoculated to an adventitious bud rooting medium for realizing elongation and rooting of the adventitious buds, in order to grow adventitious shoots. The rooting rate of the adventitious shoots reaches 90% or above, and survival rate of outside-tube rooting of adventitious shoots with roots reaches 98% or above.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture production of clustered seedlings of Clematis clematis. Background technique [0002] Clematis ( Anoectochilus roxburghii L.) is a species belonging to the genus Orchidaceae in plant taxonomy. It is widely distributed in many southern provinces from Taiwan to Yunnan in the east. It has important application and economic value as a folk herbal medicine, especially Especially in the Nanjing and Yong'an areas of Fujian Province, the production of clematis has formed a relatively large scale with an annual output value of over 1 billion. Due to the different origins and distributions of clematis, there are many obvious differences in morphology. Therefore, clematis is usually distinguished by the place of origin, for example, clematis in Taiwan, clematis in Fujian, and clematis in Yunnan. [0003] Inducing the formation of clustered buds is an im...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 杨跃生李庆玲吴鸿
Owner SOUTH CHINA AGRI UNIV
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