Exosome serving as mesenchymal stem cell source and having anti-cancer effect and medicinal preparation thereof
A bone marrow mesenchymal and exosome technology, applied in the field of biomedicine, can solve the problem of liver cancer treatment drugs not meeting clinical needs and other problems
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[0023] The substantive content of the present invention will be specifically introduced below in conjunction with the embodiments. Due to space reasons, the description of the experimental process cannot be done in great detail. All the parts not described in detail in the experiment are routine operations well known to those skilled in the art.
[0024] 1. Experimental materials
[0025] The pyridoimidazole organic compounds numbered CM1-6 are all known compounds, which were synthesized by our company's synthesizers referring to literature and conventional methods of organic synthesis, and confirmed by mass spectrometry and NMR.
[0026] RPMI1640 medium, high-glucose DMEM medium, α-MEM medium and newborn bovine serum were purchased from Gibco; human liver cancer SMMC-7721 was provided by Nanjing Medical University. BALB / C nude mice, male, 4-6 weeks old, with a body weight of 18-22 g, were provided by the Animal Experiment Center of Nanjing University. Exosome rapid extractio...
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