Method for simultaneously detecting multiple environmental hormones by SPR (surface plasma resonance) technique based on regenerated liquid optimizing

Abstract

The invention discloses a method for simultaneously detecting multiple environmental hormones by a SPR (surface plasma resonance) technique based on regenerated liquid optimizing. On the basis of an SPR sensor, an immune inhibiting method is combined; according to the difference of bonding force between different antigens and antibodies, regeneration solutions with different intensities are used for gradient elution, the SPR sensor is used for monitoring in real time, and then multiple environmental hormones can be simultaneously detected on the surface of a single-channel sensing chip. Compared with the existing surface plasma detection technique, the method has the advantages that the detection efficiency is increased by more than two times, the detection cost is reduced by more than two times, the time is shortened by more than two times, and the chip can be repeatedly used for several hundreds of times; the problem of failure to realize multiple detection due to a single channel in the traditional SPR sensor is solved; the method can be applied to accurately detecting matters with small molecular weight (smaller than 1000Da), such as bisphenol A and phthalate.

Description

technical field [0001] The invention belongs to the field of detection methods, in particular to a method for simultaneously detecting multiple environmental hormones based on surface plasmon resonance technology optimized for regeneration liquid. Background technique [0002] In recent years, environmental hormones, as hormone substances outside the biological body, have been widely used in people's daily life. Although environmental hormones have brought certain economic benefits to people, their residues in the environment and food seriously affect human health. Moreover, most environmental hormones are persistent organic pollutants, which are difficult to degrade and have certain cumulative toxicity. Therefore, the monitoring and analysis of environmental hormones is imperative. [0003] At present, environmental hormones are mostly detected and analyzed by high performance liquid chromatography, liquid chromatography-mass spectrometry, and enzyme-linked immunoassay (EL...

AI Technical Summary

Problems solved by technology

In addition, due to the variety of environmental hormones, the traditional detection process is complicated and cumbersome, and the SPR sensor with a single detection channel cannot meet the requirements of simultaneous detection of multiple environmental hormones.