Application of Wnt4 used as early biomarker of acute kidney injury in detecting early acute kidney injury

A technology for acute kidney injury and early detection, applied in the field of medicine, can solve the problem of missing the window of opportunity for treatment, and achieve the effect of high sensitivity and strong specificity

Inactive Publication Date: 2017-08-04
HARBIN MEDICAL UNIVERSITY
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  • Abstract
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Problems solved by technology

At present, the biological markers commonly used to diagnose AKI are urine output and blood creatinine level, but when the blood creatinine level rises slightly, the glomerular filtration rate has dropped by more than 50%, which indicates that the decline of glomerular function has already begun. Taking treatment has missed the "treatment opportunity window" of AKI, and the damage of renal tubular epithelial cells is difficult to be accurately assessed from the detection of serum creatinine and urine output

Method used

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  • Application of Wnt4 used as early biomarker of acute kidney injury in detecting early acute kidney injury
  • Application of Wnt4 used as early biomarker of acute kidney injury in detecting early acute kidney injury
  • Application of Wnt4 used as early biomarker of acute kidney injury in detecting early acute kidney injury

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1 Animal experiment part

[0028] Bilateral ischemia / reperfusion injury (IRI) model of C57BL / 6 mice was established: fasting but not water for 8 hours before operation, intraperitoneal injection of 1% pentobarbital 60 mg / kg, anesthetized mice. After the skin on both sides of the lower back was sheared and prepared, the surgical area was disinfected. A 1cm longitudinal incision was made on the back of both sides, and the skin, subcutaneous tissue, muscle layer, and peritoneum were opened in order to expose the renal pedicle (renal artery and vein) on the right side. After 20 minutes of the side renal artery and vein (completed within 2 minutes), the color of the kidney changes from pink to purple-black, indicating successful arterial clipping. Pay attention to the heat preservation and moisturizing of the operation area. After 20 min, the arterial clamp was released and removed, and the color of the kidney changed to pink again, indicating successful reperfus...

Embodiment 2

[0057] Embodiment 2 clinical experiment part

[0058] We conducted a clinical trial and selected patients with minimal change disease (MCD) and divided them into MCD group (MCD only) and MCD with tubular injury group (MCD with tubular injury). within the normal range. A 24-hour urine sample was obtained prior to renal biopsy. HE and Schiff periodic acid shiff (PAS) staining of paraffin sections were used to observe the pathological changes of the kidneys in each group and to score the degree of tubulointerstitial damage, and MASSON staining was used to observe the degree of fibrosis of renal tissue. Immunohistochemical staining was used to observe the expression changes of Wnt4 protein in kidney tissues of cases in each group. At the same time, Western blot and ELISA methods were used to detect (same as Example 1) the excretion of Wnt4 in the urine of patients in each group. And compared with KIM-1, a marker of renal tubular injury, to observe the relationship between the t...

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Abstract

The invention discloses application of Wnt4 used as an early biomarker of acute kidney injury in detecting early acute kidney injury. Animal experiments and clinical experiments prove that the acute kidney injury early detection biomarker Wnt4, provided by the invention, starts to rise in an early expression of acute kidney injury rats; in patients with clinical MCD combined renal tubular injury, when serum creatinine does not start to rise yet, a Wnt4 expression is remarkably increased at the injured renal tubule part, and is positively correlated with a renal tubular injury degree, so that the Wnt4 is reminded to be used as the early, non-traumatic and sensitive biomarker for detecting renal tubular injury and used for early detecting the acute kidney injury. The invention provides a novel non-traumatic acute kidney injury detection method, which is of great significance on early diagnosis and prognosis evaluation of diseases.

Description

technical field [0001] The invention relates to an early biological marker of acute kidney injury, in particular to the use of Wnt4 as an early biological marker of acute kidney injury in detecting early acute kidney injury, and the invention belongs to the technical field of medicine. Background technique [0002] The incidence of acute kidney injury (acute kidney injury, AKI) in hospitalized patients is 1%-7%, and the mortality rate is as high as 40%-60%. The rate is 50%-80%. At present, the biological markers commonly used to diagnose AKI are urine output and blood creatinine level, but when the blood creatinine level rises slightly, the glomerular filtration rate has dropped by more than 50%, which indicates that the decline of glomerular function has already begun. Taking treatment has missed the "treatment opportunity window" of AKI, and the damage of renal tubular epithelial cells is difficult to be accurately assessed from the detection of serum creatinine and urine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 李冰赵石磊
Owner HARBIN MEDICAL UNIVERSITY
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