Fluorescent quantitation calculation method based on recombinase-aid isothermal nucleic acid amplification (RAA) method

Abstract

The invention discloses a fluorescent quantitation calculation method based on a recombinase-aid isothermal nucleic acid amplification (RAA) method. A set of mathematical models and calculation methods are established for the RAA nucleic acid amplification technical features, quantitative detection can be conducted accurately to expand the initial template quantity, the RAA nucleic acid amplification technology is better applied and popularized, and the RAA technology is more rapidly, accurately and widely applied to detection and scientific research in the fields of modern agriculture, medicine, food safety, inspection and quarantine, disease control and prevention, environmental microbiological identification and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and specifically relates to a method for fluorescence quantitative calculation based on a recombinase-mediated isothermal nucleic acid amplification (RAA) method, which can accurately quantify the starting amount of nucleic acid amplification. Background technique [0002] In biological research and molecular diagnosis, DNA amplification is a basic and necessary technical means, the most commonly used of which is the use of polymerase chain reaction (English full name: Polymerase Chain Reaction, referred to as PCR) for in vitro nucleic acid amplification, This technology was invented in 1983 by Dr. Mullis of the Genetics Department of the American scientist PE (Perkin Elmer Perkin-Elmer). The Bell Prize has been widely used in modern agriculture, medicine and food industry. Through this technology, the efficient amplification of trace amounts of nucleic acid can be achieved, and a very...

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Problems solved by technology

[0020] At present, there is no isothermal nucleic acid amplification technology that can be quantified. In order to better apply and promote RAA nucleic acid amplification technology, this method was invented, so that RAA technology can be applied faster, more accurately and more widely in modern agriculture, medicine, Food safety, gun inspection and quarantine, disease control and prevention, etc.

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