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A kind of pathogen antagonist and its application

A technology of pathogenic bacteria and antagonists, applied in the field of probiotics, can solve problems such as complex inhibition mechanism

Active Publication Date: 2021-06-08
长春中俄科技园股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some probiotics have a certain inhibitory effect on pathogenic bacteria in the intestinal tract, and their inhibitory mechanism is relatively complicated

Method used

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  • A kind of pathogen antagonist and its application
  • A kind of pathogen antagonist and its application
  • A kind of pathogen antagonist and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Determination of In vitro Inhibition of Probiotic Symbionts on Representative Enteropathogenic Bacteria

[0041] The in vitro inhibition degree of single probiotic bacterium and probiotic symbiont to representative pathogenic bacteria was measured by measuring the diameter of the inhibition zone. In vitro experiments: the effect of single probiotic bacteria and probiotic symbiotic bacteria on Salmonella enterica, Shigella flexneri, Listeria monocytogenes, Staphylococcus aureus (Staphylococcus aureus), Clostridium difficile (Clostridium difficile), Escherichia coli (Escherichia coli) inhibitory effect, the specific method is as follows:

[0042] 1) Spot the activated single probiotic bacterium and probiotic symbiont on the MRS medium plate, culture anaerobically for 24 hours, and then inoculate 1% of the activated Salmonella enterica (Salmonella enterica) Bacteria to the liquid BS agar medium at about 50°C, and pour the medium quickly and evenly on the plates ...

Embodiment 2

[0061] The mixed microorganisms can maintain life activity in gastric acid, alkaline environment and human intestinal enzymes;

[0062] Detection method and results of out-of-strain tolerance of single strain and double symbiont

[0063] Probiotics must be live and colonized in the intestinal tract to exert their beneficial function. The external temperature and storage time have a direct impact on the activity of probiotics, and they must enter the intestinal tract through the oral cavity, and then reach the intestinal tract after passing through the stomach Among them, the gastric acid and pepsin in the stomach and the bile salt and trypsin in the small intestine all have antibacterial effects, which will affect the number of viable bacteria, thereby affecting the effectiveness of probiotics. Therefore, it is one of the important criteria for the screening of probiotics to consider the influence of external factors on the activity of bacteria and the passage through the di...

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Abstract

The present invention discloses a pathogenic bacteria antagonist, which includes Enterococcus durans CR-29 (Enterococcus durans CR-29) of CGMCC number 11870 and Enterococcus durans CR-29 of CGMCC number 11869. 19 (Leuconostoc mesenteroides subsp. mesenteroides SR‑19). Also disclosed is the application of the pathogenic bacteria antagonist in the preparation of drugs for preventing and / or treating diarrhea.

Description

technical field [0001] The invention belongs to the field of probiotics, and in particular relates to a pathogenic bacteria antagonist and application thereof. Background technique [0002] Salmonella, Shigella, Staphylococcus aureus, Clostridium difficile, and Listeria monocytogenes are common pathogenic bacteria in the intestinal tract of humans and animals, with strong infectivity, and a small amount of these bacteria can cause diarrhea in humans, etc. Intestinal disease, which is difficult to treat after infection by bacteria, especially Clostridium difficile. Some strains of Escherichia coli can also cause intestinal diseases, and the stability of its quantity is also of great significance to the balance of the intestinal tract. Some probiotics have a certain inhibitory effect on pathogenic bacteria in the intestinal tract, and their inhibitory mechanism is relatively complicated. In vitro studies on the inhibitory ability of a single bacterium to Clostridium difficil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/744A61P1/12A61P1/04C12R1/01
CPCA61K35/744C12N1/20C12N1/205C12R2001/01Y02A50/30
Inventor 郭聃洋王金达李永平张宇孙嘉祺
Owner 长春中俄科技园股份有限公司