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A construction method of millennium health fingerprints and its standard fingerprints

A fingerprint and construction method technology, applied in the construction method of Millennium Health fingerprint and its standard fingerprint field, can solve the problem of not being able to comprehensively evaluate the quality of medicinal materials, and achieve strong practicability, good reproducibility and precision. high effect

Active Publication Date: 2019-07-30
SICHUAN PROVINCIAL PEOPLES HOSPITAL
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Problems solved by technology

Millennium contains a large amount of volatile oil components. The 2015 edition of the Chinese Pharmacopoeia stipulates that the content of linalool in Millennium (not less than 0.2%) is used to control the quality of Millennium. However, the quality control of this single index component cannot be comprehensive Evaluate the pros and cons of medicinal materials

Method used

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  • A construction method of millennium health fingerprints and its standard fingerprints
  • A construction method of millennium health fingerprints and its standard fingerprints
  • A construction method of millennium health fingerprints and its standard fingerprints

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Embodiment 1

[0053] Construction of Millennium Health Fingerprint

[0054]1. Main instruments and reagents: Waters e2695 high performance liquid chromatograph, Waters 2998PDA detector, Empower3 chromatographic data workstation (purchased from Waters, USA); BP211D 1 / 100,000 electronic analytical balance (purchased from Sartorius, Germany); Youpu series pure water system (purchased from Chengdu Ultrapure Technology Co., Ltd.); KQ-500DB CNC ultrasonic cleaner (purchased from Kunshan Ultrasonic Instrument Co., Ltd.); standard pharmacopoeia sieve (purchased from Zhejiang Shaoxing Stainless Steel Sieve Factory); Ten batches of Qiannianjian medicinal materials provided by different manufacturers (see Table 1 below) were all identified by Professor Jiang Guihua of Chengdu University of Traditional Chinese Medicine as the dried rhizomes of the Araceae plant Homalomena occulta (Lour.) Schott; methanol and acetonitrile were chromatographically pure (purchased from DIKMA Company), phosphoric acid was ...

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Abstract

The invention discloses a method for constructing fingerprint spectra of rhizoma homalomenae and a standard fingerprint spectrum of the rhizoma homalomenae. The method includes steps of S1, precisely weighing rhizoma homalomenae powder, adding methanol into the rhizoma homalomenae powder, carrying out ultrasonic extraction, allowing extract to stand still, cooling the extract, complementing loss weights by the aid of methanol, centrifuging the extract, drying supernatant by evaporation, adding methanol into residues, re-dissolving the residues and carrying out vortex treatment and filtration to obtain subsequent filtrate which is test article solution; S2, sucking the test article solution into a liquid chromatograph and measuring the test article solution by means of gradient elution by the aid of high-performance liquid chromatography to obtain the fingerprint spectra of the rhizoma homalomenae. High-performance liquid chromatographic conditions include that octadecyl silane chemically bonded silica is used as a filler for each chromatographic column; acetonitrile is used as a mobile phase A, phosphoric acid aqueous solution is used as a mobile phase B, and a volume ratio of the mobile phase A to the mobile phase B is gradually increased along with the time; detection wavelengths are 285-305 nm. The method and the standard fingerprint spectrum have the advantages that the method is simple, convenient and stable and is high in precision and repeatability, and the method and the standard fingerprint spectrum can be used for controlling the comprehensive quality of rhizoma homalomenae medicinal materials.

Description

technical field [0001] The invention relates to a method for constructing a fingerprint of traditional Chinese medicine, in particular to a method for constructing a fingerprint of Qiannianjian and its standard fingerprint. Background technique [0002] Traditional Chinese medicine fingerprints specifically refer to the chromatogram or spectrum of a certain type or several types of components that are common to certain Chinese medicinal materials or proprietary Chinese medicines. As a comprehensive and quantifiable quality control method, the fingerprint of traditional Chinese medicine can provide more comprehensive information than a single index component. At present, FDA, British Herbal Pharmacopoeia, Indian Herbal Pharmacopoeia, German Society of Medicinal Botany, Canadian Society of Medicinal Botany, etc. all accept fingerprint quality control methods. (Provisional)". At the present stage, when most of the active ingredients of traditional Chinese medicine are not cle...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李晋奇何丹张舒涵王佳凤童荣生胡远陈璐
Owner SICHUAN PROVINCIAL PEOPLES HOSPITAL
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