Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of method for extracting scopolamine from Benzocarpus edulis

A technology of top flower bench fruit and top flower bench, which is applied in the field of natural product extraction, can solve the problems of solvents that are not environmentally friendly, poisonous to the human body, and not economical, and achieve the effects of improving the separation effect, low cost, and saving costs

Active Publication Date: 2019-10-11
XI AN JIAOTONG UNIV +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the solvent used in the current method for extracting scopolamine is not environmentally friendly, is poisonous to the human body, and has a high cost, which is not economical enough.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of method for extracting scopolamine from Benzocarpus edulis
  • A kind of method for extracting scopolamine from Benzocarpus edulis
  • A kind of method for extracting scopolamine from Benzocarpus edulis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The method for extracting scopolamine from the fruit of the top flower, comprising the following steps:

[0045] 1) Take about 7.5kg of the coarse powder of Derma chinensis, extract 3 times with 10 times the amount of 95% ethanol under reflux, each time for 2 hours, recover the extract, concentrate under reduced pressure to obtain the ethanol extract, and use 1.0kg of the extract for 10 Dissolve in 2.0% hydrochloric acid and filter. Dissolve the residue with 10 times ethyl acetate and filter. Repeat twice, 2 hours each time. Concentrate and dry the filtrate to obtain ethyl acetate extract (32.6g).

[0046]2) Take 30.0g of the extract from the ethyl acetate part of the fruit of the top flower and pass it over a normal-pressure silica gel column, and use the petroleum ether-ethyl acetate system containing 0.1% formic acid as the eluent, and the volume ratio of petroleum ether to ethyl acetate is ( The gradient of 200:1)~(0:1) was used for elution, and the eluent was colle...

Embodiment 2

[0059] The method for extracting scopolamine from the fruit of the top flower, comprising the following steps:

[0060] 1) Take about 7.5kg of the coarse powder of Derma chinensis, reflux extraction with 8 times the amount of 95% ethanol for 2 times, each time for 3 hours, recover the extract, concentrate under reduced pressure to obtain the ethanol extract, and use 1.0kg of the extract for 8 Dissolve in 1.5% hydrochloric acid and filter. Grind the filter residue with 8 times ethyl acetate and filter. Repeat for 3 times, 1 hour each time. Concentrate and dry the filtrate to obtain ethyl acetate extract (30.2 g).

[0061] 2) Take 28.0g of the extract from the ethyl acetate part of the fruit of the top flower and pass it through the silica gel column under normal pressure, use the petroleum ether-ethyl acetate system as the eluent, and the volume ratio of petroleum ether to ethyl acetate is (200:1)~ The gradient of (0:1) was eluted, and the eluent was collected in equal amounts,...

Embodiment 3

[0065] The method for extracting scopolamine from the fruit of the top flower, comprising the following steps:

[0066] 1) Take about 7.5kg of the coarse powder of the fruit of the top flower, and use 12 times the amount of 95% ethanol to reflux extract 4 times, each time for 2.5h, recover the extract, concentrate under reduced pressure to obtain the ethanol extract, and use 1.0kg of the extract 12 times the amount of 1.0% hydrochloric acid was dissolved and filtered, and the filter residue was ground and dissolved with 12 times the amount of ethyl acetate and then filtered. The number of repetitions was 4 times, each 1.5h, and the filtrate was concentrated and dried to obtain the extract of ethyl acetate (29.7g ).

[0067] 2) Take 28.0g of the extract from the ethyl acetate part of the fruit of the top flower and pass it through the silica gel column under normal pressure, use the petroleum ether-ethyl acetate system as the eluent, and the volume ratio of petroleum ether to e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for extracting scopoletin from pachysandra terminalis, belonging to the technical field of natural product extraction. The method comprises the following steps: (1) loading a sample of extract of the ethyl acetate part of the pachysandra terminalis onto a silica gel column, carrying out gradient elution by using a petroleum ether-ethyl acetate system as eluant, merging fractions and evaporating a solvent to dryness so as to obtain a first column through part; (2) taking the first column through part, loading a sample of the first column through part onto the silica gel column, carrying out gradient elution by using a formic acid-containing petroleum ether-ethyl acetate system as eluant, merging fractions having the same main spots, and then evaporating a solvent to dryness so as to obtain a second column through part; (3) loading a sample of the second column through part onto the silica gel column, carrying out gradient elution by using the formic acid-containing petroleum ether-ethyl acetate system as eluant, merging fractions having main spots, and then evaporating a solvent to dryness so as to obtain the scopoletin. The method provided by the invention is simple in operation, low in cost, little in environmental pollution and high in extracting efficiency; the purity of the extracted scopoletin reaches up to 99% or above.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting scopoletin from the fruit of the fruit of the scutellaria. Background technique [0002] Pachysandra terminalis Sieb.et Zucc. is a plant of the genus Pachysandra terminalis Sieb.et Zucc., also known as Pachysandra terminalis Sieb.et Zucc. The plant has the functions of clearing heat and detoxifying, expelling wind and dampness, relaxing tendons and activating collaterals, calming and calming the nerves. Therefore, Shaanxi folks are mainly used to treat rheumatic paralysis, numbness of limbs, bruises, menorrhagia, restlessness, etc., and can also be used as a compound medicine. Senile chronic bronchitis, rheumatoid arthritis and other diseases. So far, most of the domestic and foreign researches on Benzocarpa are limited to the reports of its steroidal alkaloids and triterpenoids, and there is no report on the extraction and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/16
Inventor 郭增军仙靓李梦怡薛璇玑张新新张卉
Owner XI AN JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com