Identification and evaluation method for canker resistance of kiwi fruit

Abstract

The invention discloses an identification and evaluation method for the canker resistance of a kiwi fruit. The condition that in vitro branch culture after inoculation treatment is carried out at a relatively low temperature and relatively high humidity is ensured through determining reasonable in vitro branch sampling time of the kiwi fruit and innovating an in vitro branch inoculation culture mode; a branch peeling mode is utilized for resistance identification; the canker resistance of the kiwi fruit is identified and evaluated through the branch browning degree, so that the identification method is simple and intuitive; the identification result and the expression of field canker resistance of the kiwi fruit have relatively high consistency; and the method is suitable for most of kiwi fruit varieties. According to the method, recognition on the canker resistance of an existing kiwi fruit variety and other wild germplasm resources is greatly promoted, a producer can select a variety with high resistance for planting according to the requirements, further development and utilization of excellent resistant germplasm resources are facilitated, and healthy and sustainable development of the whole kiwi fruit industry is promoted.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for identifying the canker resistance of kiwifruit and evaluating the resistance level by inoculating isolated branches. Background technique [0002] Actinidia bacterial canker is a devastating disease that threatens the production of kiwifruit. It is caused by Pseudomonas syringae pv. Pseudomonas syringae pv. actinidae) infection caused by bacterial diseases. Bacterial canker of kiwifruit has been found in California, USA and Shizuoka, Japan since 1980, and has become a worldwide disease. The occurrence of bacterial canker has the characteristics of wide range of growth, rapid occurrence, strong pathogenicity, and difficulty in eradication. It has been listed as a forest plant quarantine disease in my country and is a major problem in kiwifruit production. In recent years, with the continuous increase of kiwifruit cultivation area, canker disease has spread r...

AI Technical Summary

Problems solved by technology

After the occurrence of kiwifruit canker, it not only reduces the yield of kiwifruit, but also affects its fruit quality

[0003] Kiwifruit canker is a stubborn disease. Because it can infect the plant tissue, general pesticides are difficult to work. At present, there is no particularly effective control method in the world. Therefore, breeding and screening resistant kiwifruit varieties has become a disease of resistance. The best way to get rid of canker disease

However, the known kiwifruit canker resistance germplasm is very limited. One of the main reasons is that there has been a lack of effective identification and evaluation methods for kiwifruit canker resistance for a long time. Since the field observation of kiwifruit disease resistance is greatly affected by the spread of pathogenic bacteria, annual climate, and geographical environment, the field identification of disease-resistant varieties often requires a long observation period, and artificial inoculation of potted seedlings is likely to cause pathogenic bacteria. spread, and the incidence of kiwifruit canker in the seedling stage is not exactly the same as that of the adult plant, which is likely to cause large errors

Some researchers have developed the in vitro inoculation identification technology of kiwifruit isolated leaves and branches by referring to the in vitro inoculation resistance identification method of other fruit trees. It is impossible to make a more accurate and highly recognizable resistance grade division for the resistance of kiwifruit canker disease, and the error is large

The in vitro identification of kiwifruit canker disease is greatly affected by external factors such as temperature and humidity, and due to the special parasitic characteristics of canker bacteria, the in vitro identification methods used on other plants may not be suitable for kiwifruit, resulting in unsatisfactory identification results. The actual resistance in natural conditions is not the same

The immature identification and evaluation system of kiwifruit canker disease has led to relatively few reports on the identification and evaluation of resistance to kiwifruit varieties, and the level of resistance to canker disease of most varieties is unknown, which has caused difficulties in the selection of kiwifruit varieties and rational planning in production. Limiting the discovery and utilization of excellent resistant varieties

Similarly, there are excellent germplasms with high resistance to canker disease in wild kiwifruit resources, but the canker disease resistance of most kiwifruit species is not clear, which limits the development and utilization of wild kiwifruit resources with excellent resistance

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