A slicing method suitable for observing the anatomical structure of rubber tree flowers

An anatomical structure, rubber tree technology, applied in the direction of instruments, scientific instruments, test sample preparation, etc., can solve the problems that flower organs are easily corroded and dissolved, it is difficult to observe the complete structure, and the quality of slices is not easy to repeat, etc. Achieve repeatability and versatility Batch screening, good repeatability and versatility, and convenient material collection

Active Publication Date: 2020-05-05
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are three main disadvantages in the method for paraffin sectioning above: 1) There are many steps, time-consuming, easy to make mistakes in labels, and the quality of sections is not easy to repeat: the operation process of this method has more than 40 steps, and it takes 7 days from material fixing to section observation. -10 days, and when there are many materials, it is easy to mistake the label; in addition, due to the many steps, if the operation is not skilled, the slice quality is not easy to repeat; 2) For tiny particles exposed to the outside such as pollen grains and starch grains, Due to the need to replace the solution or change the container, and these steps are numerous, it is easy to cause these tiny particles to be washed off, which can not be observed on the section; The softening and corrosiveness of plant tissue are very strong, and the flower organs are easily corroded and dissolved, and it is difficult to observe the complete structure; 4) There are many volatile and toxic reagents used: in addition to alcohol, corrosive bromine and ice are also required Acetic acid, n-butanol and xylene which are somewhat toxic
In addition, multiple steps are operated at high temperature, posing safety hazards

Method used

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  • A slicing method suitable for observing the anatomical structure of rubber tree flowers
  • A slicing method suitable for observing the anatomical structure of rubber tree flowers
  • A slicing method suitable for observing the anatomical structure of rubber tree flowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the anatomical structure observation of rubber tree female flower, male flower and flower stalk

[0056] In this example, the anatomical structure of the flower organs was observed by using the spring flowers of the 9-year-old tree of the rubber tree variety CATAS 7-33-97.

[0057] Experimental conditions: In April, the inflorescences of the 9-year-old inflorescence of the rubber tree clone variety CATAS 7-33-97 were collected, and the female flowers, male flowers and flower stalks were collected respectively. The trimming method is as follows:

[0058] 1) Female flower: Pick the female flower at the top of the inflorescence and the ovary begins to develop, cut off the flower stalk with a single-sided blade, and cut off the epidermis of 0.2cm×0.2cm on the side of the enlarged ovary to form a small opening, such as figure 1 As shown, the dotted line shows the part of trimming the ovary of the female flower;

[0059] 2) Unopened male flowers: Pick the matu...

Embodiment 2

[0069] Same as Example 1, except for the following differences: the female flower incision is 0.1 cm x 0.1 cm, the unopened male flower incision is 0.1 cm x 0.3 cm, and the flower stalk is cut in lengths of 0.3 cm and 0.8 cm.

Embodiment 3

[0071] Same as Example 1, except for the following differences: the fixative is 2wt% paraformaldehyde and 5wt% glutaraldehyde in 0.01M phosphate buffer, pH 7.2; the first wash process is 0.005M phosphate buffer, pH 7.8 , the soaking time is 10min; the second time is a mixed solution of two-thirds of 0.005M pH 7.8 phosphate buffer and one-third of the OCT embedding medium, the soaking time is 20min; the third time is the OCT embedding medium , soaking time 40min.

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Abstract

A slicing method suitable for observing rubber tree floral organ anatomical structure includes following steps: (1), putting one or several of rubber tree female flowers, male flowers and flower stalks which are cropped into fixing liquid, extracting air, and fixing for a period of time; (2), sequentially using phosphate buffer, a mixed solution of the phosphate buffer and an OCT embedding agent and the OCT embedding agent to rinse a material obtained in the step (1), and using the OCT embedding agent to embed a frozen slice; (3), slicing the embedded material obtained in the step (2), sticking the slice, sealing the slice, and observing. No volatile and toxic reagent is used in the method, and the method is simple and quick in operation and high in repeatability and universality.

Description

technical field [0001] The invention belongs to the technical field of plant histochemistry, and relates to a method for slicing plant tissue, in particular to a method for slicing the anatomical structure of rubber tree flowers. Background technique [0002] Rubber tree is a panicle, which is born in leaf axils or scale axils, monoecious, and dimorphic flowers; female flowers are born at the top of pedicels, usually each inflorescence has 3-20 female flowers, and the base of female flowers has a green disc; ovary The upper position, the style is short or absent, the stigma is attached to the ovary, the central axis of the placenta, usually 3 cells, each cell has a side ovule; the male flowers are smaller than the female flowers, the number is more, no green disc, no petals, calyx base connate, calyx 5-toothed or 5-parted, beige, stamens 5-10, arranged in two whorls and connate on the frontal stigma, there is a lot of pollen in the drug chamber. Rubber tree seed trees bloom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/36
CPCG01N1/286G01N1/36G01N2001/2873
Inventor 张世鑫田维敏
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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