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Method for Screening Molecular Markers Related to Leaf Growth of Duckweed Duckweed

A purple-backed duckweed, molecular marker technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of fast detection and improved biological treatment efficiency

Active Publication Date: 2020-07-24
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection method of the invention has the advantages of rapidity, accuracy, sensitivity, etc., and can intuitively detect the genotypes of different individuals of the rusty spot, so as to quickly obtain the polymorphism map of the genetic variation of the functional gene microsatellite locus of the rusty spot, the microsatellite Markers can be applied to the fields of genetic variation and population genetic diversity analysis of rust spot moth, but there is still room for improvement in the detection speed of microsatellite markers

Method used

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  • Method for Screening Molecular Markers Related to Leaf Growth of Duckweed Duckweed
  • Method for Screening Molecular Markers Related to Leaf Growth of Duckweed Duckweed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The method for screening the molecular markers related to the growth of Duckweed leaves includes clone culture, genomic DNA extraction, microsatellite primer design, PCR amplification, and data statistics and analysis.

[0017] The composition and concentration of the nutrient solution for plant clone culture are: nitrogen 0.3~0.4g / L, phosphoric anhydride 0.1~0.15 g / L, potassium oxide 0.16~0.23 g / L, magnesium oxide 0.01~0.015 g / L and sulfur 0.012 ~0.02 g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of the purple-backed duckweed, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0018] The plant clones were cultured indoors in a light incubator for 50-70 days, the culture conditions were 22.5-23.5°C, the humidity was 73-83%, the light intensity was 1800-220...

Embodiment 2

[0023] The method for screening the molecular markers related to the growth of Duckweed leaves includes clone culture, genomic DNA extraction, microsatellite primer design, PCR amplification, and data statistics and analysis.

[0024] The composition and concentration of the nutrient solution for plant clone cultivation are: nitrogen 0.32 g / L, phosphoric anhydride 0.12 g / L, potassium oxide 0.19 g / L, magnesium oxide 0.012 g / L and sulfur 0.016 g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of the purple-backed duckweed, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0025] The plant clones were cultivated indoors for 60 days in a light incubator, the culture conditions were 23°C, humidity 78%, light intensity 2000lux, and light-dark time 16:8h. Under the above c...

Embodiment 3

[0030] The method for screening the molecular markers related to the growth of Duckweed leaves includes clone culture, genomic DNA extraction, microsatellite primer design, PCR amplification, and data statistics and analysis.

[0031] The composition and concentration of the nutrient solution for plant clone cultivation are: nitrogen 0.32g / L, phosphoric anhydride 0.12g / L, potassium oxide 0.2g / L, magnesium oxide 0.013g / L and sulfur 0.017g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of the purple-backed duckweed, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0032] Count the number of leaves for each plant (connected leaves) in different culture lines, count the number of roots for each leaf, and measure the leaf length (longest leaf length), leaf width (shorte...

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Abstract

The invention discloses a method for screening a molecular marker associated with the growth of spirodela polyrrhiza leaves, which includes clone culture, genome DNA extraction, microsatellite primer design, PCR (Polymerase Chain Reaction) amplification and data statistics and analysis. The method has the following beneficial effects: the method can be used for rapidly obtaining a large quantity of DNA sequences of microsatellite primer-marked spirodela polyrrhiza, the speed of microsatellite marker assay is high, and a polymorphic map of genetic marker loci Sp25 and Sp30 of spirodela polyrrhiza showing high genetic variation can be rapidly obtained. The leaf length of spirodela polyrrhiza is obviously associated with the locus Sp30 and the leaf width is obviously associated with the locus Sp25 (P is less than 0.05), and spirodela polyrrhiza leaves in which the genotype of the locus Sp25 is CE have the largest form and are in the largest number.

Description

technical field [0001] The invention relates to the technical field of DNA molecular markers, in particular to a method for screening molecular markers related to the growth of purple-backed duckweed leaves. Background technique [0002] Rich, highly heterozygous and good stability, co-dominant inheritance following Mendelian segregation law, easy PCR amplification, etc., have become widely used DNA markers in recent years, and are often used in family pedigree authentication and gene linkage analysis of biological resources , genetic map construction, germplasm identification, population genetic diversity and many other research fields. [0003] In the natural environment, Duckweed basically reproduces asexually, and when the environmental conditions are suitable, one generation can be cloned in 2 days, and the genetic diversity of the population is low. [0004] The prior art provides a variety of detection methods for satellite markers, for example, Chinese Invention Aut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐娜娜徐嘉俊朱旭辉
Owner ZHEJIANG OCEAN UNIV
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